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Titolo:
cDNA-AFLP analysis of differential gene expression in the prokaryotic plant pathogen Erwinia carotovora
Autore:
Dellagi, A; Birch, PRJ; Heilbronn, J; Lyon, GD; Toth, IK;
Indirizzi:
Scottish Crop Res Inst, Dept Fungal & Bacterial Plant Pathol, Dundee DD2 5DA, Scotland Scottish Crop Res Inst Dundee Scotland DD2 5DA Dundee DD2 5DA, Scotland
Titolo Testata:
MICROBIOLOGY-UK
, volume: 146, anno: 2000,
parte:, 1
pagine: 165 - 171
SICI:
1350-0872(200001)146:<165:CAODGE>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSPOSON MUTAGENESIS; SUBSP ATROSEPTICA; PCR DETECTION; RNA; DISPLAY; TISSUE;
Keywords:
prokaryotic differential gene expression; Erwinia; plant pathogen; cDNA-AFLP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Toth, IK Scottish Crop Res Inst, Dept Fungal & Bacterial Plant Pathol, Dundee DD2 5DA, Scotland Scottish Crop Res Inst Dundee Scotland DD2 5DA D2 5DA, Scotland
Citazione:
A. Dellagi et al., "cDNA-AFLP analysis of differential gene expression in the prokaryotic plant pathogen Erwinia carotovora", MICROBIO-UK, 146, 2000, pp. 165-171

Abstract

For studies or differential gene expression In prokaryotes, methods Tor Bacterial Plant Pathology, Synthesizing representative cDNA populations are required, Here, a technique Scottish Crop Research Institute, , is describedfor the synthesis of cDNA from the potato pathogens Erwinia carotovora subsp, atroseptica (Eca) and Erwinia carotovora subsp. carotovora (Ecc) using a combination of short oligonucleotide (Il-mer) primers that were known to anneal to conserved sequences in the 3' regions of enterobaderial genes, Specific PCR amplifications with primers designed to anneal to 14 known genesfrom either Eca or Ecc revealed the presence of the corresponding transcripts in cDNA, suggesting that the cDNA represented a broad genomic coverage,cDNA-amplified fragment length polymorphism (cDNA-AFLP) was used to identify differentially expressed genes in Eca. including one that shows significant similarity, at the protein level, to an avirulence gene from Xanthomonas campestris pv, raphani, Northern analysis was used to confirm that differentially amplified cDNA fragments were derived from differentially expressed genes, This is the first report of the use of cDNA-AFLP to study differential gene expression in prokaryotes.

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Documento generato il 24/09/20 alle ore 04:43:12