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Titolo:
Therapeutic oral vaccination induces mucosal immune response sufficient toeliminate long-term Helicobacter pylori infection
Autore:
Ikewaki, J; Nishizono, A; Goto, T; Fujioka, T; Mifune, K;
Indirizzi:
Oita Med Univ, Dept Microbiol, Oita 8795593, Japan Oita Med Univ Oita Japan 8795593 iv, Dept Microbiol, Oita 8795593, Japan Oita Med Univ, Dept Internal Med 2, Oita 8795593, Japan Oita Med Univ Oita Japan 8795593 ept Internal Med 2, Oita 8795593, Japan
Titolo Testata:
MICROBIOLOGY AND IMMUNOLOGY
fascicolo: 1, volume: 44, anno: 2000,
pagine: 29 - 39
SICI:
0385-5600(2000)44:1<29:TOVIMI>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHOLERA-TOXIN; BALB/C MICE; TH2 CELLS; IMMUNIZATION; UREASE; FELIS; INDUCTION; MODEL;
Keywords:
therapeutic vaccination; Helicobacter pylori; secretory IgA; th2 cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Nishizono, A Oita Med Univ, Dept Microbiol, Idaigaoka 1-1,Hasama Machi, Oita 8795593, Japan Oita Med Univ Idaigaoka 1-1,Hasama Machi Oita Japan 8795593
Citazione:
J. Ikewaki et al., "Therapeutic oral vaccination induces mucosal immune response sufficient toeliminate long-term Helicobacter pylori infection", MICROB IMMU, 44(1), 2000, pp. 29-39

Abstract

We examined the efficacy of therapeutic oral vaccination using Helicobacter pylori-whole cell sonicate and cholera toxin (CT) in mice persistently infected with H. pylori. Efficacy was determined by bacterial culture and microscopic examination of gastric tissues for the persistence of bacteria at 6 weeks after the last vaccination. Vaccination of H. pylori-whole cell sonicate combined with CT eradicated bacteria in 10/16 mice (62.5%). Interestingly, oral vaccination with CT alone also eliminated the bacteria in 8/17 mice (47.1%). However, a therapeutic intraperitoneally administered vaccine failed to eradicate H. pylori from the stomach (1/17 mice, 5.9%). Identification of the type of immunity involved in the eradication process showed that oral vaccination enhanced the antigen-specific IgA in the feces and saliva. The efficacy of eradication of H. pylori correlated well with increasesin IgA secretion in mucosal tissue and a higher labeling index of IgA-positive lumina of pyloric glands. Moreover, the expression of IL-1 mRNA in thestomach of mice with eradicated bacteria was higher than in the uneradicated group. Our results suggest that the efficacy of vaccination depends on the mucosal IgA response in the gastrointestinal tract against H. pylori viaTh2 cell activation and that therapeutic oral vaccination induces a mucosal immune response sufficient to eradicate long-term infection with H. pylori.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 13:19:52