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Titolo:
Neonatal neutrophil interaction with P-selectin: contribution of P-selectin glycoprotein ligand-1 and sialic acid
Autore:
Tcharmtchi, MH; Smith, CW; Mariscalco, MM;
Indirizzi:
Baylor Coll Med, CNRC, Dept Pediat, Sect Leukocyte Biol, Houston, TX 77030USA Baylor Coll Med Houston TX USA 77030 Leukocyte Biol, Houston, TX 77030USA Baylor Coll Med, Dept Pediat, Sect Crit Care Med, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 Crit Care Med, Houston, TX 77030 USA
Titolo Testata:
JOURNAL OF LEUKOCYTE BIOLOGY
fascicolo: 1, volume: 67, anno: 2000,
pagine: 73 - 80
SICI:
0741-5400(200001)67:1<73:NNIWPC>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
LEUKOCYTE ADHESION MOLECULE-1; EPIDERMAL GROWTH-FACTOR; ENDOTHELIAL-CELLS; CD18-INDEPENDENT ADHESION; CHEMOTACTIC FACTORS; TYROSINE SULFATION; AMINO-TERMINUS; MYELOID CELLS; SHEAR-STRESS; IN-VITRO;
Keywords:
human; cell trafficking; adhesion molecules; ontogeny;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Mariscalco, MM Baylor Coll Med, CNRC, Dept Pediat, Sect Leukocyte Biol, 1100 Bates,Room 6014, Houston, TX 77030 USA Baylor Coll Med 1100 Bates,Room 6014 Houston TX USA 77030 A
Citazione:
M.H. Tcharmtchi et al., "Neonatal neutrophil interaction with P-selectin: contribution of P-selectin glycoprotein ligand-1 and sialic acid", J LEUK BIOL, 67(1), 2000, pp. 73-80

Abstract

Previously we had determined that neonatal neutrophils had decreased interaction with monolayers expressing P-selectin compared to adult cells. In this study we examined the function of neonatal P-selectin glycoprotein ligand-l (PSGL-1), A rabbit polyclonal antibody directed against the amino terminus of human PSGL-1 was produced and purified (3RB-PSGL-1), Neonatal neutrophils expressed the epitope recognized by 3RB-PSGL-1 and expression was decreased compared with adult neutrophils (20%, P < 0.05). In addition neonatal neutrophils had decreased interaction with Chinese hamster ovary (CHO)-P-selectin under both shear conditions and static adhesion (P < 0.05), Treatment of both neonatal and adult neutrophils with 3RB-PSGL-1 similarly inhibited the interaction with P-selectin monolayers under shear conditions, effects similar to treatment with O-sialoglycoprotein endopeptidase (OSGE). Neuraminidase treatment of neonatal and adult cells also markedly inhibited the interaction. In a detachment assay marked differences were noted between neonatal and adult cells treated with either 3RB-PSGL-1 or neuraminidase. Such treatments had little effect on adult neutrophils until shear stress exceeded 2.8 dynes/cm(2). Treated neonatal neutrophils were exquisitely sensitive to shear stress with a marked decrease in interaction noted at a shearstress as low as 0.6 dynes/cm(2). Thus the adhesive mechanisms that remainafter treatment with neuraminidase or 3RB-PSGL-1 have a relatively low avidity and function less well in neonatal neutrophils compared to adult neutrophils, We speculate that this may account for the less efficient adhesion of neonatal neutrophils to P-selectin under conditions of flow.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/21 alle ore 04:11:37