Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Effects of thermodynamic nonideality in protein crystal growth
Autore:
Grant, ML;
Indirizzi:
Yale Univ, Dept Chem Engn, New Haven, CT 06520 USA Yale Univ New Haven CTUSA 06520 Dept Chem Engn, New Haven, CT 06520 USA
Titolo Testata:
JOURNAL OF CRYSTAL GROWTH
fascicolo: 1, volume: 209, anno: 2000,
pagine: 130 - 137
SICI:
0022-0248(200001)209:1<130:EOTNIP>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOMER CONCENTRATIONS; LYSOZYME SOLUTIONS; CONSTANTS; PH;
Keywords:
proteins; thermodynamics; activity coefficients; supersaturation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
--discip_EC--
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Grant, ML Yale Univ, Dept Chem Engn, POB 208286, New Haven, CT 06520 USA Yale Univ POB 208286 New Haven CT USA 06520 Haven, CT 06520 USA
Citazione:
M.L. Grant, "Effects of thermodynamic nonideality in protein crystal growth", J CRYST GR, 209(1), 2000, pp. 130-137

Abstract

The driving force for crystallization is the difference in chemical potential between the protein in supersaturated solution and at its solubility. This chemical potential difference depends in a complicated manner on protein concentration and solution conditions (temperature, ionic strength and pH). Consequently, driving forces estimated from the nominal ideal solution supersaturation may be in error. In this work, the chemical potential of protein in solution is estimated from a virial expansion in protein concentration. The thermodynamic driving force for the crystallization of hen egg white lysozyme is calculated as a function of temperature, salt concentration,and protein concentration. In all cases examined here, the driving force for crystallization is lower than that for the corresponding ideal solution and the deviation from ideal solution behavior increases with the protein solubility. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 09:32:25