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Titolo:
Depletion of glutathione as a cause of the promotive effects of polygodial, a sesquiterpene on the production of reactive oxygen species in Saccharomyces cerevisiae
Autore:
Machida, K; Tanaka, T; Taniguchi, M;
Indirizzi:
Osaka City Univ, Dept Bio & Geosci, Grad Sch Sci, Sumiyoshi Ku, Osaka 5588585, Japan Osaka City Univ Osaka Japan 5588585 , Sumiyoshi Ku, Osaka 5588585, Japan
Titolo Testata:
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
fascicolo: 5, volume: 88, anno: 1999,
pagine: 526 - 530
SICI:
1389-1723(199911)88:5<526:DOGAAC>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
MITOCHONDRIA; ARREST;
Keywords:
polygodial; glutathione; reactive oxygen species; mitochondria; Saccharomyces cerevisiae;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Tanaka, T Osaka City Univ, Dept Bio & Geosci, Grad Sch Sci, Sumiyoshi Ku, 3-3-138 Sugimoto, Osaka 5588585, Japan Osaka City Univ 3-3-138 Sugimoto Osaka Japan 5588585 585, Japan
Citazione:
K. Machida et al., "Depletion of glutathione as a cause of the promotive effects of polygodial, a sesquiterpene on the production of reactive oxygen species in Saccharomyces cerevisiae", J BIOSCI BI, 88(5), 1999, pp. 526-530

Abstract

The pungent sesquiterpenoid unsaturated dialdehyde, polygodial, exhibited a strong yeastcidal activity against the cells of Saccharomyces cerevisiae,in which production of reactive oxygen species (ROS) at a significant level could be detected with a fluorescent probe. The production of ROS in polygodial-treated cells was further confirmed by its elimination and the accompanying protection against yeastcidal effects in the presence of antioxidants such as L-ascorbate and alpha-tocopherol (alpha-TOH). Polygodial could accelerate ROS production only in cells of the wild-type grande strain but not in those of the respiratory-deficient petite mutant (rho(0)), indicatingthe role of the mitochondrial electron transport chain in the production of ROS. Unlike the case with antimycin A which accelerates ROS production bydirectly targeting the mitochondrial electron dow, polygodial caused depletion of cytoplasmic and mitochondrial glutathione which functions in eliminating ROS inevitably generated during aerobic growth. Polygodial-mediated depletion of intracellular glutathione was possibly dependent on a direct interaction between its enal moiety and the sulfhydryl group of the cysteine in glutathione by a Michael-type reaction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 09:17:06