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Titolo:
Evaluation of phenotypic and molecular typing techniques for determining diversity in Erwinia carotovora subspp. atroseptica
Autore:
Toth, IK; Bertheau, Y; Hyman, LJ; Laplaze, L; Lopez, MM; McNicol, J; Niepold, F; Persson, P; Salmond, GPC; Sletten, A; van der Wolf, JM; Perombelon, MCM;
Indirizzi:
Scottish Crop Res Inst, Dundee DD2 5DA, Scotland Scottish Crop Res Inst Dundee Scotland DD2 5DA Dundee DD2 5DA, Scotland INRA, INA PG, Paris, France INRA Paris FranceINRA, INA PG, Paris, France IVIA, Valencia, Spain IVIA Valencia SpainIVIA, Valencia, Spain Biomath & Stat Scotland, Scottish Crop Res Inst, Dundee, Scotland Biomath & Stat Scotland Dundee Scotland Crop Res Inst, Dundee, Scotland Biol Bundesanstalt Braunschweig, Braunschweig, Germany Biol Bundesanstalt Braunschweig Braunschweig Germany unschweig, Germany Swedish Univ Agr Sci, Dept Plant Pathol, Uppsala, Sweden Swedish Univ Agr Sci Uppsala Sweden Dept Plant Pathol, Uppsala, Sweden Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England Univ Cambridge Cambridge England CB2 1QW hem, Cambridge CB2 1QW, England Norwegian Crop Res Inst, Dept Plant Pathol, Aas, Norway Norwegian Crop ResInst Aas Norway Inst, Dept Plant Pathol, Aas, Norway IPO, DLO, Res Inst Plant Protect, NL-6700 AA Wageningen, Netherlands IPO Wageningen Netherlands NL-6700 AA NL-6700 AA Wageningen, Netherlands
Titolo Testata:
JOURNAL OF APPLIED MICROBIOLOGY
fascicolo: 5, volume: 87, anno: 1999,
pagine: 770 - 781
SICI:
1364-5072(199911)87:5<770:EOPAMT>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
FRAGMENT-LENGTH-POLYMORPHISM; PLANT-PATHOGENIC BACTERIA; REPETITIVE DNA-SEQUENCES; GENERALIZED TRANSDUCTION; MONOCLONAL-ANTIBODIES; POTATO STRAINS; IDENTIFICATION; CHRYSANTHEMI; SYSTEM; SEROGROUPS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Toth, IK Scottish Crop Res Inst, Dundee DD2 5DA, Scotland Scottish Crop Res Inst Dundee Scotland DD2 5DA D2 5DA, Scotland
Citazione:
I.K. Toth et al., "Evaluation of phenotypic and molecular typing techniques for determining diversity in Erwinia carotovora subspp. atroseptica", J APPL MICR, 87(5), 1999, pp. 770-781

Abstract

A number of phenotypic and molecular fingerprinting techniques, including physiological profiling (Biolog), restriction fragment length polymorphism (RFLP), enterobacterial repetitive intergenic consensus (ERIC) and a phage typing system, were evaluated for their ability to differentiate between 60strains of Erwinia carotovora ssp. atroseptica (Eca) from eight west European countries. These techniques were compared with other fingerprinting techniques, random amplified polymorphic DNA (RAPD) and Ouchterlony double diffusion (ODD), previously used to type this pathogen. Where possible, data were represented as dendrograms and groups/subgroups of strains identified. Simpson's index of diversity (Simpson's D) was used to compare groupings obtained with the different techniques which, with the exception of Biolog, gave values of 0.46 (RFLP), 0.39 (ERIC), 0.83 (phage typng), 0.82 (RAPD) and0.26 (ODD). Of the techniques tested, phage typing showed the highest level of diversity within Eca, and this technique will now form the basis of studies into the epidemiology of blackleg disease.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 03:28:23