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Titolo:
Identification of novel serine proteinase gene transcripts in the midguts of two tropical insect pests, Scirpophaga incertulas (Wk.) and Helicoverpa armigera (Hb.)
Autore:
Mazumdar-Leighton, S; Babu, CR; Bennett, J;
Indirizzi:
Univ Delhi, Dept Bot, Delhi 110007, India Univ Delhi Delhi India 110007Univ Delhi, Dept Bot, Delhi 110007, India Int Rice Res Inst, Plant Breeding Genet & Biochem Div, Los Banos, Philippines Int Rice Res Inst Los Banos Philippines hem Div, Los Banos, Philippines
Titolo Testata:
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
fascicolo: 1, volume: 30, anno: 2000,
pagine: 57 - 68
SICI:
0965-1748(200001)30:1<57:IONSPG>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUBSTRATE-SPECIFICITY; PLODIA-INTERPUNCTELLA; LEPIDOPTERAN LARVAE; PROTEASE GENES; TRYPSIN; INHIBITORS; SEQUENCE; CDNAS; EXPRESSION; ADAPTATION;
Keywords:
serine proteinase; trypsin; midgut; cDNA; RT PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Mazumdar-Leighton, S Cornell Univ, Dept Entomol, Geneva, NY 14456 USA Cornell Univ Geneva NY USA 14456 eneva, NY 14456 USA
Citazione:
S. Mazumdar-Leighton et al., "Identification of novel serine proteinase gene transcripts in the midguts of two tropical insect pests, Scirpophaga incertulas (Wk.) and Helicoverpa armigera (Hb.)", INSEC BIO M, 30(1), 2000, pp. 57-68

Abstract

We have used RT PCR and 3'RACE to identify diverse serine proteinase genesexpressed in the midguts of the rice yellow stem borer (Scirpophaga incertulas) and Asian corn borer (Helicoverpa armigera). The RT-PCR primers encoded the conserved regions around the active site histidine57 and serine195 of Drosophila melanogaster a trypsin, including aspartate189 of the specificity pocket. These primers amplified three transcripts (SiP1-3) from midgutsof S. incertulas, and two transcripts (HaP1-2) from midguts of H. armigera. The five RT PCR products were sequenced to permit design of gene-specificforward primers for use with anchored oligo dT primers in 3'RACE. Sequencing of the 3'RACE products indicated that SiP1, SiP2 and HaP1 encoded trypsin-like serine proteinases, while HaP2 encoded a chymotrypsin-like serine -proteinases. The SiP3 transcript proved to be an abundant 960 nt mRNA encoding a trypsin-like protein in which the active site serinel95 was replaced by aspartate. The possible functions of this unusual protein are discussed. (C) 2000 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/01/21 alle ore 17:14:06