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Titolo:
Hypomorphic bimA(APC3) alleles cause errors in chromosome metabolism ghat activate the DNA damage checkpoint blocking cytokinesis in Aspergillus nidulans
Autore:
Wolkow, TD; Mirabito, PM; Venkatram, S; Hamer, JE;
Indirizzi:
Purdue Univ, Dept Biol, W Lafayette, IN 47907 USA Purdue Univ W LafayetteIN USA 47907 Dept Biol, W Lafayette, IN 47907 USA Univ Kentucky, Sch Biol Sci, Mol & Cellular Biol Sect, Lexington, KY 40506USA Univ Kentucky Lexington KY USA 40506 ar Biol Sect, Lexington, KY 40506USA
Titolo Testata:
GENETICS
fascicolo: 1, volume: 154, anno: 2000,
pagine: 167 - 179
SICI:
0016-6731(200001)154:1<167:HBACEI>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANAPHASE-PROMOTING COMPLEX; CELL-CYCLE; S-PHASE; SEPTUM FORMATION; FISSION YEAST; SACCHAROMYCES-CEREVISIAE; TYR15 PHOSPHORYLATION; NUCLEAR DIVISION; PROTEIN MAD2; SNAP HELIX;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Wolkow, TD Harvard Univ, Sch Med, Dept Genet, 200 Longwood Ave, Boston, MA02115 USA Harvard Univ 200 Longwood Ave Boston MA USA 02115 MA 02115 USA
Citazione:
T.D. Wolkow et al., "Hypomorphic bimA(APC3) alleles cause errors in chromosome metabolism ghat activate the DNA damage checkpoint blocking cytokinesis in Aspergillus nidulans", GENETICS, 154(1), 2000, pp. 167-179

Abstract

The Aspergillus nidulans sepI(+) gene has been implicated in the coordination of septation with nuclear division and cell growth. We find that the temperature-sensitive (ts) sepI1 mutation represents a novel allele of bimA(APC3), which encodes a conserved component of the anaphase-promoting complex/cyclosome (APC/C). We have characterized the septation, nuclear division, cell-cycle checkpoint defects, and DNA sequence alterations of sepI1 (renamed bimA10) and two other ts lethal bimA(APC3) alleles, bimA1 and bimA9. Ourobservations that bimA9 and bimA10 strains had morphologically abnormal nuclei, chromosome segregation defects, synthetic phenotypes with mutations in the DNA damage checkpoint genes uvsB(MEC1/rad3) or uvsD(+), and enhanced sensitivity to hydroxyurea strongly suggest that these strains accumulate errors in DNA metabolism. We found that the aseptate phenotype of bimA9 and bimA10 strains was substantially relieved by mutations in uvsB(MEC1/rad3) or uvsD(+), suggesting that rile presence of a functional DNA damage checkpoint inhibits septation in these bimA(APC3) strains. Our results demonstratethat mutations in bimA(APC3) lead to errors in DNA metabolism that indirectly block septation.

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Documento generato il 06/04/20 alle ore 05:19:47