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Titolo:
Human granulocytic ehrlichiosis agent infection in a pony vaccinated with a Borrelia burgdorferi recombinant OspA vaccine and challenged by exposure to naturally infected ticks
Autore:
Chang, YF; McDonough, SP; Chang, CF; Shin, KS; Yen, W; Divers, T;
Indirizzi:
Cornell Univ, Coll Vet Med, Dept Popular med & Diagnost Sci, Ithaca, NY 14853 USA Cornell Univ Ithaca NY USA 14853 med & Diagnost Sci, Ithaca, NY 14853 USA Cornell Univ, Coll Vet Med, Dept Biomed Sci, Ithaca, NY 14853 USA Cornell Univ Ithaca NY USA 14853 d, Dept Biomed Sci, Ithaca, NY 14853 USA Cornell Univ, Coll Vet Med, Dept Clin Sci, Ithaca, NY 14853 USA Cornell Univ Ithaca NY USA 14853 Med, Dept Clin Sci, Ithaca, NY 14853 USA
Titolo Testata:
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
fascicolo: 1, volume: 7, anno: 2000,
pagine: 68 - 71
SICI:
1071-412X(200001)7:1<68:HGEAII>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEW-YORK-STATE; LYME-DISEASE; IXODES-SCAPULARIS; BORNE PATHOGENS; DEER TICKS; EQUI; IXODIDAE; HORSES; ACARI; TRANSMISSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Chang, YF Cornell Univ, Coll Vet Med, Dept Popular med & Diagnost Sci, Ithaca, NY 14853 USA Cornell Univ Ithaca NY USA 14853 nost Sci, Ithaca, NY 14853 USA
Citazione:
Y.F. Chang et al., "Human granulocytic ehrlichiosis agent infection in a pony vaccinated with a Borrelia burgdorferi recombinant OspA vaccine and challenged by exposure to naturally infected ticks", CL DIAG LAB, 7(1), 2000, pp. 68-71

Abstract

A pony was vaccinated with recombinant OspA vaccine (rOspA) and then exposed 3 months later to Borrelia burgdorferi-infected ticks (Ixodes scapularis) collected in Westchester County, N.Y. At 2 weeks after tick exposure, thepony developed a high fever (105 degrees F), Buffy coat smears showed that20% of neutrophils contained ehrlichial inclusion bodies (morulae). Flunixin Meglumine (1 g daily) was given for 2 days, and the body temperature returned to normal. PCR for ehrlichial DNA was performed an blood samples for 10 consecutive days beginning when the pony was first febrile. This pony was monitored for another 3.5 months but developed no further clinical signs. The 44-kDa immunodominant human granulocytic ehrlichiosis antigen gene wasamplified by PCR and cloned into a pCR2.1 vector. DNA sequence analysis ofthis gene showed it was only 8 bp different (99% identity) from the results reported by others (J.W. Ijdo et al., Infect. Immun. 66:3264-3269, 1998),Western blot analysis, growth inhibition assays, and repeated attempts to isolate B. burgdorferi all demonstrated the pony was protected against B. burgdorferi infection. These results highlight the potential for ticks to harbor and transmit several pathogens simultaneously, which further complicates the diagnosis and vaccination of these emerging tick-borne diseases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 11:42:18