Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CD38 ligation inhibits normal and leukemic myelopoiesis
Autore:
Todisco, E; Suzuki, T; Srivannaboon, K; Coustan-Smith, E; Raimondi, SC; Behm, FG; Kitanaka, A; Campana, D;
Indirizzi:
St Jude Childrens Res Hosp, Dept Hematol Oncol, Memphis, TN 38105 USA St Jude Childrens Res Hosp Memphis TN USA 38105 ol, Memphis, TN 38105 USA St Jude Childrens Res Hosp, Dept Pathol, Memphis, TN 38105 USA St Jude Childrens Res Hosp Memphis TN USA 38105 ol, Memphis, TN 38105 USA Univ Tennessee, Coll Med, Memphis, TN 38163 USA Univ Tennessee Memphis TNUSA 38163 ssee, Coll Med, Memphis, TN 38163 USA Kagawa Med Univ, Dept Internal Med 1, Kagawa, Japan Kagawa Med Univ Kagawa Japan d Univ, Dept Internal Med 1, Kagawa, Japan
Titolo Testata:
BLOOD
fascicolo: 2, volume: 95, anno: 2000,
pagine: 535 - 542
SICI:
0006-4971(20000115)95:2<535:CLINAL>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC STEM-CELLS; ACUTE LYMPHOBLASTIC-LEUKEMIA; ACUTE MYELOID-LEUKEMIA; SURFACE ANTIGEN CD38; ADP-RIBOSYL CYCLASE; C-CBL PROTOONCOGENE; BONE-MARROW; TYROSINE PHOSPHORYLATION; PHOSPHATIDYLINOSITOL 3-KINASE; MONOCLONAL-ANTIBODY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Campana, D St Jude Childrens Res Hosp, Dept Hematol Oncol, 332 N Lauderdale St, Memphis, TN 38105 USA St Jude Childrens Res Hosp 332 N Lauderdale St Memphis TN USA 38105
Citazione:
E. Todisco et al., "CD38 ligation inhibits normal and leukemic myelopoiesis", BLOOD, 95(2), 2000, pp. 535-542

Abstract

CD38 is a transmembrane molecule whose expression varies during hematopoietic cell differentiation. We used stroma-supported cultures of human myeloid cells to assess the effects of CD38 ligation on myeloid differentiation. In 8 experiments with CD34(+) cells purified from normal bone marrow or cord blood, flow cytometry used with antibodies to CD34 and myeloperoxidase (MPO) identified 4 cell populations after 7 days of culture, Addition of anti-CD38 (T16) to the cultures induced a profound reduction of the most mature(CD34(-)MPO(++)) cell population, which includes promyelocytes, myelocytesand metamyelocytes; mean (+/- SD) cell recovery was 12.8% +/- 9.8% of thatin parallel cultures with an isotype-matched control antibody. The suppressive effect of CD38 ligation on phenotypically more immature normal cells was inconsistent but generally less pronounced, Recovery of CD34(++)MPO(-) cells was 63.3% +/- 24.4%, recovery of CD34([+/-]) MPO- cells was 95.3% +/- 35.1%, and recovery of CD34(-)MPO(+) cells was 42.0% +/- 18.7% of that in control cultures. However, anti-CD38 suppressed recovery of cells obtained from 6 patients with CD38(+) acute myeloid leukemia; after 7-day cultures, cell recovery was 25.2% +/- 21.7% of that in control cultures. Cell recoverywas also reduced by F(ab')(2) or Fab fragments of anti-CD38. CD38 ligationdramatically suppressed recovery of murine 32D myeloid cells transfected with human CD38 and cocultured with stroma (3.8% +/- 7.3%; n = 7). CD38 ligation of CD38(+) 32D cells also induced cell aggregation, tyrosine kinase activity, and Ca++ influx. We conclude that CD38 mediates signals that culminate in suppression of myeloid cell growth and survival, (Blood, 2000;95:535-542) (C) 2000 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 16:09:37