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Titolo:
Transient reactivation of CSF in parthenogenetic one-cell mouse embryos
Autore:
Ciemerych, MA; Kubiak, JZ;
Indirizzi:
Univ Warsaw, Inst Zool, Dept Embryol, PL-00927 Warsaw, Poland Univ WarsawWarsaw Poland PL-00927 Dept Embryol, PL-00927 Warsaw, Poland Univ Paris 06, CNRS, UMR 7622, F-75005 Paris, France Univ Paris 06 ParisFrance F-75005 CNRS, UMR 7622, F-75005 Paris, France
Titolo Testata:
BIOLOGY OF THE CELL
fascicolo: 9, volume: 91, anno: 1999,
pagine: 641 - 647
SICI:
0248-4900(199912)91:9<641:TROCIP>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
METAPHASE-II ARREST; PROTO-ONCOGENE PRODUCT; MAP KINASE; MEIOTIC MATURATION; XENOPUS-OOCYTES; PROTOONCOGENE PRODUCT; CYCLIN-B; EGGS; MOS; ACTIVATION;
Keywords:
cell cycle; MAP kinase; meiosis; mitosis; protein phosphorylation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Kubiak, JZ Univ Rennes 1, CNRS, UPR 41,2 Ave Prof Leon Bernard, F-35043 Rennes, France Univ Rennes 1 UPR 41,2 Ave Prof Leon Bernard Rennes France F-35043
Citazione:
M.A. Ciemerych e J.Z. Kubiak, "Transient reactivation of CSF in parthenogenetic one-cell mouse embryos", BIO CELL, 91(9), 1999, pp. 641-647

Abstract

During meiosis, the cytostatic factor (CSF) activity stabilizes the activity of the M-phase promoting factor (MPF) in metaphase II arrested vertebrate oocytes. Upon oocyte activation, the inactivation of both MPF and CSF enables the entry into the first embryonic mitotic cell cycle. Using a biological assay based on cell-fusion (hybrid between a parthenogenetically activated egg entering the first mitotic division and an activated oocyte), we observed that in activated mouse oocytes a first drop in CSF activity is detectable as early as 20 min post-activation. This suggests that CSF is inactivated upon MPF inactivation. However, CSF activity increases again to reacha maximum 60 min postactivation and gradually disappears during the following 40 min. Thus, in activated mouse oocytes (undergoing the transition to interphase) CSF activity fluctuates before definitive inactivation. We found that hybrids arrested in M-phase, thus containing CSF activity after oocyte activation, have activated forms of MAP kinases while hybrids in interphase have inactive forms of these enzymes. We postulate that CSF inactivation in mouse oocytes proceeds in two steps. The initial inactivation of CSF, required for MPF inactivation, is transient and does not require MAP kinaseinactivation. The final inactivation of CSF, required for normal embryoniccell cycle progression, is dependent upon the inactivation of MAP kinases. (C) 1999 Editions scientifiques et medicales Elsevier SAS.

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Documento generato il 29/11/20 alle ore 05:44:40