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Titolo:
Selection of an immunogenic peptide mimic of the capsular polysaccharide of Neisseria meningitidis serogroup A using a peptide display library
Autore:
Grothaus, MC; Srivastava, N; Smithson, SL; Kieber-Emmons, T; Williams, DB; Carlone, GM; Westerink, MAJ;
Indirizzi:
Med Coll Ohio, Dept Pathol & Med, Toledo, OH 43699 USA Med Coll Ohio Toledo OH USA 43699 Dept Pathol & Med, Toledo, OH 43699 USA Univ Penn, Dept Pathol & Lab Med, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 & Lab Med, Philadelphia, PA 19104 USA Ctr Dis Control, Div Bacterial & Mycot Dis, Resp Dis Branch, Atlanta, GA 30333 USA Ctr Dis Control Atlanta GA USA 30333 sp Dis Branch, Atlanta, GA 30333 USA
Titolo Testata:
VACCINE
fascicolo: 13, volume: 18, anno: 2000,
pagine: 1253 - 1263
SICI:
0264-410X(20000118)18:13<1253:SOAIPM>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN CONJUGATE VACCINE; RANDOMIZED CONTROLLED TRIAL; IMMUNOLOGICAL MEMORY; ANTICARBOHYDRATE ANTIBODIES; MENINGOCOCCAL MENINGITIS; FILAMENTOUS PHAGE; INDUCTION; LIGANDS; INFANTS; EPIDEMIC;
Keywords:
meningococcal group A vaccine; peptide mimicry; phage display;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Westerink, MAJ Med Coll Ohio, Dept Pathol & Med, POB 10008, Toledo, OH 43699 USA Med Coll Ohio POB 10008 Toledo OH USA 43699 o, OH 43699 USA
Citazione:
M.C. Grothaus et al., "Selection of an immunogenic peptide mimic of the capsular polysaccharide of Neisseria meningitidis serogroup A using a peptide display library", VACCINE, 18(13), 2000, pp. 1253-1263

Abstract

The presently available meningococcal vaccine is poorly immunogenic in infants and fails to induce long-lasting immunity in adults. Efforts to convert this TI-2 type vaccine into a T dependent vaccine ape being actively pursued and include conjugate vaccine development. Alternatively, the meningococcal polysaccharide can be rendered into a T dependent antigen through the use of peptides which mimic the capsular polysaccharide complexed or conjugated to potent protein carrier molecules. We have previously developed an anti-idiotypic monoclonal antibody (mAb) based peptide mimic of meningococcal group C polysaccharide (MCPS). A direct approach to identification of peptide mimics of antigen is through the use of peptide display libraries. We have utilized a phage library and a mAb with specificity for meningococcal group A polysaccharide (MAPS) to screen for a peptide mimic of MAPS. Six different peptide motifs were selected with the use of the mAb. Thirty-eight of the 60 sequenced phage clones were represented by motif 1 and 2 which differed only in three amino acids at the carboxy terminus. Immunological assays were performed. Phage clones with motif 1 and 2 were capable of bindinghuman hyperimmune sera and inhibiting the binding of human hyperimmune sera to nominal antigen. Immunization with motif 1 peptide complexed to proteosomes resulted in an anti-MAPS antibody response. Priming with the peptide proteosome complex induced an anamnestic response indicating the formation of immunological memory. (C) 2000 Elsevier Science Ltd. All rights reserved.

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Documento generato il 29/09/20 alle ore 08:35:03