Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Transduction of dendritic cell progenitors with a retroviral vector encoding viral interleukin-10 and enhanced green fluorescent protein allows purification of potentially tolerogenic antigen-presenting cells
Autore:
Takayama, T; Tahara, H; Thomson, AW;
Indirizzi:
Univ Pittsburgh, Med Ctr, Dept Surg, Thomas E Starzl Transplantat Inst, Pittsburgh, PA 15213 USA Univ Pittsburgh Pittsburgh PA USA 15213 at Inst, Pittsburgh, PA 15213 USA Univ Pittsburgh, Med Ctr, Dept Mol Genet & Biochem, Pittsburgh, PA 15213 USA Univ Pittsburgh Pittsburgh PA USA 15213 Biochem, Pittsburgh, PA 15213 USA
Titolo Testata:
TRANSPLANTATION
fascicolo: 12, volume: 68, anno: 1999,
pagine: 1903 - 1909
SICI:
0041-1337(199912)68:12<1903:TODCPW>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDIATED GENE-TRANSFER; MAJOR HISTOCOMPATIBILITY COMPLEX; INHIBITS CYTOKINE PRODUCTION; CARDIAC ALLOGRAFT SURVIVAL; COLONY-STIMULATING FACTOR; HEMATOPOIETIC-CELLS; BONE-MARROW; TOLERANCE INDUCTION; IL-10; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Thomson, AW Univ Pittsburgh, Med Ctr, Dept Surg, Thomas E Starzl Transplantat Inst, W1544 Biomed Sci Tower,200 Lothrop St, Pittsburgh, PA 15213 USA Univ Pittsburgh W1544 Biomed Sci Tower,200 Lothrop St Pittsburgh PA USA 15213
Citazione:
T. Takayama et al., "Transduction of dendritic cell progenitors with a retroviral vector encoding viral interleukin-10 and enhanced green fluorescent protein allows purification of potentially tolerogenic antigen-presenting cells", TRANSPLANT, 68(12), 1999, pp. 1903-1909

Abstract

Background. Dendritic cells (DC) are important antigen-presenting cells that play critical roles in the initiation and modulation of immune responses. Genetic engineering of DC to express immunosuppressive molecules is a novel approach to the inhibition of allograft rejection, Retroviral delivery of viral interleukin (vIL)-10 to replicating myeloid DC progenitors (DCp) impairs their T-cell stimulatory capacity and promotes the induction of antigen-specific T-cell hyporesponsiveness, However, transduction efficiency with retroviral vectors is comparatively low. Enhanced green fluorescent protein (EGFP) is important both as a marker of gene transduction and for the selection of transduced cells. Our aims were to construct a retroviral vectorencoding both vIL-10 and EGFP, to positively select transduced DC, and to assess the impact of these highly purified, vIL-10-secreting antigen-presenting cells on allogeneic T-cell responses,Methods. DCp propagated from bone marrow of C57BL10 (H2(b)) mice in granulocyte-macrophage colony-stimulating factor (GM-CSF)+IL-4 were transduced with a retroviral vector encoding both vIL-10 and EGFP by centrifugal enhancement. Gene transfer efficiency was determined by flow cytometry. Transducedcells were flow sorted, and vIL-10 secretion was quantified by ELISA, DC function was assessed by the ability of the cells to induce naive allogeneic(C3H; H2(k)) T-cell proliferation and cytotoxic T lymphocyte generation. Results. Retrovirally transduced DC expressed both vIL-10 and EGFP gene products. Approximately 20% of unsorted cells expressed EGFP, as determined by flow cytometry, vIL-10 was produced at a mean rate of 31 ng/40 hr/10(6) cells. After sorting, the incidence of EGFP(+) DC was increased dramaticallyto at least 95%, and the production of vIL-10 was increased approximately three- to fourfold, to a mean of 107 ng/40 hr/10(6) cells. These highly purified, vIL-10-secreting DC exhibited markedly diminished capacity to induceallogeneic T-cell proliferative and cytotoxic responses. Conclusions. DCp retrovirally transduced to express both vIL-10 and EGFP can be rapidly identified and sorted to high levels of purity. The availability of highly enriched preparations of vIL-10-transduced DC facilitates studies of their immunoregulatory function and may enhance their therapeutic potential in transplantation or autoimmune disease.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 19:42:48