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Titolo:
Effects of recombinant thrombopoietin on the growth of murine primitive and committed hematopoietic progenitors in serum-free culture
Autore:
Sasaki, H; Ikuta, K; Funabiki, T; Fujioka, KI; Matsuda, M;
Indirizzi:
Yokohama City Univ, Sch Med, Dept Pediat, Yokohama, Kanagawa 2360004, Japan Yokohama City Univ Yokohama Kanagawa Japan 2360004 anagawa 2360004, Japan
Titolo Testata:
PEDIATRICS INTERNATIONAL
fascicolo: 6, volume: 41, anno: 1999,
pagine: 666 - 672
SICI:
1328-8067(199912)41:6<666:EORTOT>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
C-MPL LIGAND; MEGAKARYOCYTE COLONY FORMATION; STEM-CELL FACTOR; PLATELET PRODUCTION; IN-VITRO; INTERLEUKIN-3; PROLIFERATION; MICE; STIMULATION; RECEPTOR;
Keywords:
hematopoietic progenitors; high proliferative potential colony forming cells; serum-free culture; thrombopoietin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Sasaki, H Yokohama City Univ, Sch Med, Dept Pediat, 3-9 Fukuura, Yokohama,Kanagawa 2360004, Japan Yokohama City Univ 3-9 Fukuura Yokohama Kanagawa Japan 2360004 n
Citazione:
H. Sasaki et al., "Effects of recombinant thrombopoietin on the growth of murine primitive and committed hematopoietic progenitors in serum-free culture", PEDIATR INT, 41(6), 1999, pp. 666-672

Abstract

Background: The aim of the present study was to determine the effect of thrombopoietin (Tpo) in combination with other cytokines on the growth of murine megakaryocytic, granulocyte-macrophage, erythroid and primitive hematopoietic progenitor cells, excluding possibilities of synergistic effects by serum factor(s). Methods: Serum-free clonogenic assay systems were used for assay of colonyforming units in megakaryocytes (CFU-Mk), colony forming units in granulocytes-macrophages (CFU-GM), burst-forming units in erythrocytes (BFU-E) in marrow of normal mice and of high proliferative potential colony forming cells in 5-fluorouracil-treated marrow. Results: Serum-free culture enabled megakaryocyte colony growth by recombinant murine (rm) Tpo and this was synergistically supported with rm interleukin (IL)-3, rm stem cell factor (SCF) or recombinant human (rh) erythropoietin (Epo). Recombinant human IL-6, rhIL-11 and rm granulocyte-macrophage colony stimulating factor (GM-CSF) showed synergistic effects with rmTpo only in the presence of serum. Recombinant murine IL-3 or rmSCF increased the large colonies and mixed-type colonies containing other populations, suggesting that these cytokines promoted the proliferation of immature populations of CFU-Mk. The rmTpo enhanced the growth of granulocyte-macrophage (GM) colonies stimulated by rmGM-CSF or rmIL-3 and erythroid bursts by rhEpo, with or without rmIL-3. The rmTpo also significantly increased HPP colonies insynergy with rmIL-3 only in the presence of serum or rmSCF. Conclusion: Serum-free culture is valuable for evaluating synergistic effects of cytokines and Tpo acts not only on megakaryocytic progenitors but also on granulocyte-macrophage, erythroid and primitive progenitor cells in combination with other cytokines, such as rmIL-3 and rmSCF. However, serum or SCF was required for enhancement of the colony growth of primitive progenitors by Tpo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 09:46:08