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Titolo:
Identification of heterologous translocation partner genes fused to the BCL6 gene in diffuse large B-cell lymphomas: 5 '-RACE and LA-PCR analyses of biopsy samples
Autore:
Yoshida, S; Kaneita, Y; Aoki, Y; Seto, M; Mori, S; Moriyama, M;
Indirizzi:
Univ Tokyo, Inst Med Sci, Dept Pathol, Tokyo, Japan Univ Tokyo Tokyo Japan v Tokyo, Inst Med Sci, Dept Pathol, Tokyo, Japan Aichi Canc Inst, Lab Chemotherapy, Nagoya, Aichi, Japan Aichi Canc Inst Nagoya Aichi Japan ab Chemotherapy, Nagoya, Aichi, Japan Tottori Univ, Fac Med, Sch Life Sci, Dept Mol Biol, Tottori 6838503, JapanTottori Univ Tottori Japan 6838503 Dept Mol Biol, Tottori 6838503, Japan
Titolo Testata:
ONCOGENE
fascicolo: 56, volume: 18, anno: 1999,
pagine: 7994 - 7999
SICI:
0950-9232(199912)18:56<7994:IOHTPG>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
NON-HODGKINS-LYMPHOMA; FINGER ENCODING GENE; SOMATIC HYPERMUTATION; BURKITT-LYMPHOMA; CHROMOSOMAL BREAKPOINT; 3Q27 TRANSLOCATION; MOLECULAR-CLONING; ONCOGENE; LOCUS; PROTEIN;
Keywords:
BCL6; translocation; hypermutation; lymphoma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Moriyama, M Tottori Univ, Fac Med, Sch Life Sci, Dept Mol Biol, 86 Nishimachi, Tottori6838503, Japan Tottori Univ 86 Nishimachi Tottori Japan 6838503 38503, Japan
Citazione:
S. Yoshida et al., "Identification of heterologous translocation partner genes fused to the BCL6 gene in diffuse large B-cell lymphomas: 5 '-RACE and LA-PCR analyses of biopsy samples", ONCOGENE, 18(56), 1999, pp. 7994-7999

Abstract

In order to elucidate the molecular mechanism(s) for BCL6 translocation, we identified translocational partner genes by subjecting clinical biopsy samples from patients with non-Hodgkin's lymphoma to 5'-rapid amplification of cDNA ends (5'-RACE). Sequence analysis of the 5'-RACE product revealed that the BCL6 gene was fused to the J segment of the immunoglobulin heavy chain (IgH) gene in about half of the cases, but in the other half, it was fused to heterologous partners, including the MHC class II transactivator (CIITA), pim-1, eukaryotic initiation factor 4AII (ejf4AII), transferrin receptor (TFRR) and ikaros genes, Since analyses using genomic long and accurate (LA)-PCR revealed that the breakpoints in the partner gene mere confined tothe first intron or the second exon in all cases, the promoter and the first exon of the BCL6 gene mere replaced by the promoter and the first or both the first and second exon of the partner gene, The breakpoint Banking sequences had no recombination signal sequences (RSSs) or chi sequences and were homologous with the switch region only when the BCL6 gene was fused to the IgH gene, suggesting that BCL6 translocation cannot be explained solely by mistakes of V(D)J, or chi-mediated or class-switch recombination, but rather another mechanism may also be required to explain the molecular mechanism for the promiscuous BCL6 translocation.

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Documento generato il 01/12/20 alle ore 00:56:46