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Titolo:
CD138/syndecan-1: A useful immunohistochemical marker of normal and neoplastic plasma cells on routine trephine bone marrow biopsies
Autore:
Chilosi, M; Adami, F; Lestani, M; Montagna, L; Cimarosto, L; Semenzato, G; Pizzolo, G; Menestrina, F;
Indirizzi:
Univ Verona, Dept Pathol, Sect Anat Pathol, Policlin Borgo Roma, I-37134 Verona, Italy Univ Verona Verona Italy I-37134 iclin Borgo Roma, I-37134 Verona, Italy Univ Padua, Dept Clin & Expt Med, Clin Immunol Sect, I-35100 Padua, Italy Univ Padua Padua Italy I-35100 , Clin Immunol Sect, I-35100 Padua, Italy Univ Verona, Dept Clin & Expt Med, Sect Hemathol, I-37100 Verona, Italy Univ Verona Verona Italy I-37100 d, Sect Hemathol, I-37100 Verona, Italy
Titolo Testata:
MODERN PATHOLOGY
fascicolo: 12, volume: 12, anno: 1999,
pagine: 1101 - 1106
SICI:
0893-3952(199912)12:12<1101:CAUIMO>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNOGLOBULIN LIGHT-CHAINS; MULTIPLE-MYELOMA; MONOCLONAL-ANTIBODY; I COLLAGEN; IMMUNOPEROXIDASE TECHNIQUE; IMMUNOBLASTIC LYMPHOMA; DIAGNOSTIC-CRITERIA; SYNDECAN-1; EXPRESSION; DIFFERENTIATION;
Keywords:
CD138; immunohistochemistry; lymphoma; myeloma; plasma cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Chilosi, M Univ Verona, Dept Pathol, Sect Anat Pathol, Policlin Borgo Roma, I-37134 Verona, Italy Univ Verona Verona Italy I-37134 Roma, I-37134 Verona, Italy
Citazione:
M. Chilosi et al., "CD138/syndecan-1: A useful immunohistochemical marker of normal and neoplastic plasma cells on routine trephine bone marrow biopsies", MOD PATHOL, 12(12), 1999, pp. 1101-1106

Abstract

Detection of abnormal numbers and/or distribution of bone marrow (BM) plasma cells (PCs) on trephine biopsies can be important in the differential diagnosis of multiple myeloma (MM) and other PC disorders. A variety of immunohistochemical markers can potentially improve the specificity and sensitivity of PC detection on routine histological sections obtained from trephineBM biopsies, but most of them are not completely satisfactory. In this study, we investigated whether the antibody CD138/B-B4, which is an optimal marker for PC detection on BM aspirates by flow cytometry, can be used successfully for the identification of PCs also on formalin-fixed, decalcified biopsies. A series of samples including normal BM [12], MM [65], monoclonal gammopathies of undetermined significance [44], and B-cell lymphoma of various types [94], including B-cell precursor lymphoblastic leukemia [9], lymphoplasmacytoid [17], immunoblastic [14], lymphocytic/CLL [23], hairy cell leukemia [4], large B-cell [8], mantle-cell [3], marginal zone [6] and follicular [10] lymphomas, have been investigated for CD138 expression using a sensitive immunohistochemical technique. Within the BM microenvironment, CD138 was characterized by excellent sensitivity and specificity. Virtually allnormal and neoplastic PCs expressed clear-cut membrane CD138 immunostaining, whereas all other cell types did not. All cases of MM, including plasmablastic and leukemic cases, showed strong immunoreactivity, Conversely, all B-cell lymphomas, including all cases characterized by secretive features, lymphoplasmacytoid, and immunoblastic lymphomas, were completely negative. These results demonstrate that CD138 is a highly sensitive and specific marker that is useful for the rapid and precise localization of normal and neoplastic PCs on routine BM sections. In addition, because of its clear-cut cell membrane localization, CD138 can be used successfully in double-marker immunostaining reactions to evaluate precisely nuclear prognostic markers such as Ki67 and p53 in MMs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 03:10:19