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Titolo:
Initial evidence for the involvement of bone morphogenetic protein-2 earlyduring periosteal chondrogenesis
Autore:
Sanyal, A; Sarkar, G; Saris, DBF; Fitzsimmons, JS; Bolander, ME; ODriscoll, SW;
Indirizzi:
Mayo Clin & Mayo Fdn, Cartilage & Connect Tissue Res Lab, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn Rochester MN USA 55905 Lab, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn, Dept Orthoped, Mol Biol Lab, Rochester, MN 55905 USAMayo Clin & Mayo Fdn Rochester MN USA 55905 Lab, Rochester, MN 55905 USA
Titolo Testata:
JOURNAL OF ORTHOPAEDIC RESEARCH
fascicolo: 6, volume: 17, anno: 1999,
pagine: 926 - 934
SICI:
0736-0266(199911)17:6<926:IEFTIO>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-BETA; CHICK LIMB; MESSENGER-RNA; CELL-SHAPE; TGF-BETA; CARTILAGE DIFFERENTIATION; ARTICULAR-CARTILAGE; PATTERN-FORMATION; GENE-EXPRESSION; CHONDROCYTES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: O'Driscoll, SW Mayo Clin & Mayo Fdn, Cartilage & Connect Tissue Res Lab, 200 1st St SW, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn 200 1st St SW Rochester MN USA 55905 A
Citazione:
A. Sanyal et al., "Initial evidence for the involvement of bone morphogenetic protein-2 earlyduring periosteal chondrogenesis", J ORTHOP R, 17(6), 1999, pp. 926-934

Abstract

The potential of periosteum to form cartilage makes periosteal transplantation a viable approach to repairing defects in articular cartilage, which has a limited potential for repair. However, cartilage repair, including that by periosteal chondrogenesis, is poorly understood. Consequently, a thorough understanding of its molecular mechanisms will help to achieve the quality of neocartilage required for its clinical application in damaged joints. An in vitro model was used to study the early molecular events of periosteal chondrogenesis. During the search for the expression of transforming growth factor-beta-related mRNAs in this model system. bone morphogenetic protein-2 mRNA expression was found to be upregulated 20-fold within the first12 hours of culture. This stimulation was dependent on the explants being suspended in agarose and did not occur with explants cultured in liquid medium. The upregulation of bone morphogenetic protein-2 mRNA expression was also enhanced by exogenously added transforming growth factor-beta 1 in the presence of fetal calf serum. The upregulation, however, was not transient:rather, it persisted over a prolonged period in both transforming growth factor-beta 1-treated and untreated explants. Further data indicate that this stimulation of bone morphogenetic protein-2 mRNA expression was regulatedat the transcriptional level and that no new protein synthesis was required for this. Bone morphogenetic protein-2 is known to influence developmental chondrogenesis; therefore, these observations direct our attention towardan important potential role of it as a regulator of the early events in cartilage repair. Furthermore, because periosteum produces fracture (cartilage) callus, these findings may be important in defining the molecular mechanisms of fracture healing.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 04:11:58