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Titolo:
Wild-type and mutant forms of v-sre differentially alter neuronal migration and differentiation in vivo
Autore:
Morgan, JC; Majors, JE; Galileo, DS;
Indirizzi:
Med Coll Georgia, Dept Cellular Biol & Anat, Augusta, GA 30912 USA Med Coll Georgia Augusta GA USA 30912 Biol & Anat, Augusta, GA 30912 USA Washington Univ, Sch Med, Dept Biochem & Mol Biophys, St Louis, MO USA Washington Univ St Louis MO USA Biochem & Mol Biophys, St Louis, MO USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE RESEARCH
fascicolo: 2, volume: 59, anno: 2000,
pagine: 226 - 237
SICI:
0360-4012(20000115)59:2<226:WAMFOV>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
ROUS-SARCOMA VIRUS; CHICKEN OPTIC TECTUM; CLONALLY RELATED CELLS; AMINO-TERMINAL DOMAIN; NEURITE OUTGROWTH; DEVELOPING BRAIN; FAMILY KINASES; ADHESION; PROTEIN; MICE;
Keywords:
v-src; tyrosine kinase; optic tectum; retroviral vector; chick embryo;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Galileo, DS Med Coll Georgia, Dept Cellular Biol & Anat, Augusta, GA 30912USA Med Coll Georgia Augusta GA USA 30912 , Augusta, GA 30912 USA
Citazione:
J.C. Morgan et al., "Wild-type and mutant forms of v-sre differentially alter neuronal migration and differentiation in vivo", J NEUROSC R, 59(2), 2000, pp. 226-237

Abstract

The effects of three different forms of v-src on brain cell development were determined in vivo. Recombinant retroviral vectors encoding the marker lacZ (control) and either wild-type v-src or SH2 or SH3 domain-deleted formsof v-src (Delta SH2 or Delta SH3, respectively) were used to infect neuronal progenitor cells in the embryonic chicken midbrain (optic tectum; OT). Embryos were injected in the OT with retroviral concentrates on embryonic day (E) 3 and sacrificed at E6, E9, and later in development. Patterns of cell proliferation, migration, and differentiation of lacZ-marked clonal cell progeny were then analyzed. Relative to lacZ-only controls, cell clone sizeat E6 was significantly increased for v-src-, unchanged for Delta SH2-, and smaller for Delta SH3-injected embryos. At E9, Delta SH2 cell clones weresignificantly larger than controls, suggesting increased survival from normal programmed cell death. Radial neuronal migration was impaired for v-srcand Delta SH3 clones, whereas tangential neuronal migration was enhanced along fiber tracts in v-src and Delta SH2 clones. Moreover, radial glial cell development and differentiation was hindered in v-src and Delta SH3 clones. These experiments demonstrate that ectopic v-src signaling alters proliferation, migration, survival, and differentiation of developing brain cellsand suggest that src signaling pathways are involved in these developmental processes. Furthermore, certain effects of v-src on brain cells require specific src homology domains. (C) 2000 Wiley-Liss, Inc.

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Documento generato il 05/04/20 alle ore 05:36:26