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Titolo:
AMPA-kainate subtypes of glutamate receptor in rat cerebral microglia
Autore:
Noda, M; Nakanishi, H; Nabekura, J; Akaike, N;
Indirizzi:
Kyushu Univ, Grad Sch Med Sci, Lab Cellular & Syst Physiol, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 & Syst Physiol, Fukuoka 8128582, Japan Kyushu Univ, Fac Dent, Dept Pharmacol, Fukuoka 8128582, Japan Kyushu UnivFukuoka Japan 8128582 Dept Pharmacol, Fukuoka 8128582, Japan
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 1, volume: 20, anno: 2000,
pagine: 251 - 258
SICI:
0270-6474(20000101)20:1<251:ASOGRI>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXCITATORY AMINO-ACIDS; GLIAL-CELLS; NEUROTRANSMITTER-RELEASE; CULTURED ASTROCYTES; HIPPOCAMPAL-NEURONS; CA2+ PERMEABILITY; CONCANAVALIN-A; CHANNELS; CALCIUM; BRAIN;
Keywords:
microglia; whole-cell patch clamp; kainate; glutamate; AMPA; cyclothiazide; reverse transcription-PCR; tumor necrosis factor-alpha;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
65
Recensione:
Indirizzi per estratti:
Indirizzo: Akaike, N Kyushu Univ, Grad Sch Med Sci, Lab Cellular & Syst Physiol, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 siol, Fukuoka 8128582, Japan
Citazione:
M. Noda et al., "AMPA-kainate subtypes of glutamate receptor in rat cerebral microglia", J NEUROSC, 20(1), 2000, pp. 251-258

Abstract

Microglial cells were isolated from rat cerebral cortex, and kainate (KA)-induced inward current was measured at a holding potential of -40 or -60 mV. 6-Cyano-7-nitroquinoxaline-2, 3-dione-sensitive KA-induced currents increased with increasing KA concentration. The half-activation concentration and Hill coefficient were 3.3 x 10(-4) M and 1.4, respectively. Although glutamate (Glu) and AMPA-induced currents were much smaller than that induced by KA, all KA-, Glu-, and AMPA-induced currents were greatly and consistently enhanced in the presence of cyclothiazide (CTZ). On the other hand, KA- induced currents were much less sensitive to potentiation by concanavain A, suggesting that the KA-induced response in rat microglia is predominantly mediated by AMPA-preferring receptors (subunits GluR1-GluR4). The current-voltage relationships of KA- and AMPA-CTZ-induced currents were almost linearor slightly outward rectifying. The reversal potential of KA- induced current shifted to negative potentials (from 14 to -40 mV) on switching from high Na+ to high Ca2+ external solution, indicating the low Ca2+ permeabilitythrough the AMPA-KA receptor channel complexes. AMPA-KA receptor expression was studied with immunohistochemistry and reverse transcription-PCR, fromwhich GluR2, GluR3, GluR4, and GluR5 were identified. Lower levels of mRNAs for GluR7 and KA-1-KA-2 were also indicated. Finally, activation of thesereceptors with KA or Glu significantly enhanced the production of tumor necrosis factor-alpha. These results suggest that primary cultured rat microglia possesses functional Glu receptor, which may mediate neuron to microglia communication in the physiological and pathological states.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 13:48:41