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Titolo:
AMPA receptor calcium permeability, GluR2 expression, and selective motoneuron vulnerability
Autore:
Vandenberghe, W; Robberecht, W; Brorson, JR;
Indirizzi:
Univ Chicago, Dept Neurol, Chicago, IL 60637 USA Univ Chicago Chicago IL USA 60637 ago, Dept Neurol, Chicago, IL 60637 USA Univ Louvain, Dept Neurol, B-3000 Louvain, Belgium Univ Louvain Louvain Belgium B-3000 Dept Neurol, B-3000 Louvain, Belgium
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 1, volume: 20, anno: 2000,
pagine: 123 - 132
SICI:
0270-6474(20000101)20:1<123:ARCPGE>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT SPINAL-CORD; AMYOTROPHIC-LATERAL-SCLEROSIS; CEREBELLAR PURKINJE-CELLS; SUBUNIT MESSENGER-RNAS; IN-SITU HYBRIDIZATION; CA2+ PERMEABILITY; GLUTAMATE RECEPTORS; RECTIFICATION PROPERTIES; DIFFERENTIAL EXPRESSION; HIPPOCAMPAL-NEURONS;
Keywords:
amyotrophic lateral sclerosis; excitotoxicity; kainate; dorsal horn; rat; culture;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Brorson, JR Univ Chicago, Dept Neurol, MC2030,5841 S Maryland Ave, Chicago, IL 60637 USA Univ Chicago MC2030,5841 S Maryland Ave Chicago IL USA 60637A
Citazione:
W. Vandenberghe et al., "AMPA receptor calcium permeability, GluR2 expression, and selective motoneuron vulnerability", J NEUROSC, 20(1), 2000, pp. 123-132

Abstract

AMPA receptor-mediated excitotoxicity is proposed to play a major pathogenic role in the selective motoneuron death of amyotrophic lateral sclerosis. Motoneurons have been shown in various models to be more susceptible to AMPA receptor-mediated injury than other spinal neurons. It has been hypothesized that this selective vulnerability of motoneurons is caused by the expression of highly Ca2+-permeable AMPA receptors and a complete or relative lack of the AMPA receptor subunit Glu receptor 2 (GluR2). The aim of this study was to quantify the relative Ca2+ permeability of AMPA receptors and the fractional expression of GluR2 in motoneurons by combining whole-cell patch-clamp electrophysiology and single-cell RT-PCR and to compare these properties with those of dorsal horn neurons. Spinal motoneurons and dorsal horn neurons were isolated from embryonic rats and cultured on spinal astrocytes. As in previous studies, motoneurons were significantly more vulnerable to AMPA and kainate than dorsal horn neurons. However, all motoneurons expressed GluR2 mRNA (similar to 40% of total AMPA receptor subunit mRNA), and their AMPA receptors had intermediate whole-cell relative Ca2+ permeability(PCa2+/PCs+ similar to 0.4). AMPA receptor PCa2+/PCs+ and the relative abundance of GluR2 varied more widely in dorsal horn neurons than in motoneurons, but the mean values did not differ significantly between the two cell populations. GluR2 was virtually completely edited at the Q/R site both in motoneurons and dorsal horn neurons. These results indicate that the selective vulnerability of motoneurons to AMPA receptor agonists is not determinedsolely by whole-cell relative Ca2+ permeability of AMPA receptors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 10:33:09