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Titolo:
HTLV-I specific IFN-gamma+CD8+lymphocytes correlate with the proviral loadin peripheral blood of infected individuals
Autore:
Kubota, R; Kawanishi, T; Matsubara, H; Manns, A; Jacobson, S;
Indirizzi:
NINDS, Viral Immunol Sect, Bethesda, MD 20892 USA NINDS Bethesda MD USA 20892 S, Viral Immunol Sect, Bethesda, MD 20892 USA NCI, Expt Immunol Branch, NIH, Bethesda, MD 20892 USA NCI Bethesda MD USA20892 xpt Immunol Branch, NIH, Bethesda, MD 20892 USA NCI, Viral Epidemiol Branch, NIH, Bethesda, MD 20892 USA NCI Bethesda MD USA 20892 l Epidemiol Branch, NIH, Bethesda, MD 20892 USA
Titolo Testata:
JOURNAL OF NEUROIMMUNOLOGY
fascicolo: 2, volume: 102, anno: 2000,
pagine: 208 - 215
SICI:
0165-5728(20000124)102:2<208:HSICWT>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
VIRUS TYPE-I; TROPICAL SPASTIC PARAPARESIS; CELL LEUKEMIA-VIRUS; CYTOTOXIC T-LYMPHOCYTES; CENTRAL-NERVOUS-SYSTEM; SPINAL-CORD LESIONS; MYELOPATHY HAM-TSP; NEUROLOGICAL DISEASE; CEREBROSPINAL-FLUID; SURFACE PHENOTYPE;
Keywords:
human T lymphotropic virus type I (HTLV-I); HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP); proviral load; CD8+lymphocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Jacobson, S NINDS, Viral Immunol Sect, 9000 Rockville Pike, Bethesda, MD 20892 USA NINDS 9000 Rockville Pike Bethesda MD USA 20892 , MD 20892 USA
Citazione:
R. Kubota et al., "HTLV-I specific IFN-gamma+CD8+lymphocytes correlate with the proviral loadin peripheral blood of infected individuals", J NEUROIMM, 102(2), 2000, pp. 208-215

Abstract

Human T lymphotropic virus type I (HTLV-I)-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is an inflammatory neurological disease caused by HTLV-I infection. It has been shown that HAM/TSP patients have high proviral loads and an extraordinarily high frequency of circulating CD8 + cytotoxic T lymphocytes specific for HTLV-I in their peripheral blood when compared to asymptomatic HTLV-I carriers (AC). We have previously described anintracellular cytokine detection assay, in which interferon-gamma (IFN-gamma)+ CD8 + lymphocytes are specific for HTLV-I in infected individuals, Here, we have established a competitive polymerase chain reaction assay to measure the proviral load of patients and investigate a potential relationshipbetween proviral load and virus-specific CD8 + lymphocytes. Genomic DNA was extracted from peripheral blood lymphocytes (PBL) from eight HAM/TSP patients and seven AC for the measurement of HTLV-I measuring proviral loads, The same PBL were analyzed for intracellular IFN-gamma expression by flow cytometry. In the HAM/TSP patients and AC, the average proviral lends were 34,482 and 9784 copy/mu g DNA (P = 0.021), and the average of IFN-gamma + CD8+ lymphocytes in total PBL were 1.47 and 0.08% (P = 0.001), respectively. It was confirmed that HAM/TSP patients have both high proviral loads and increased HTLV-I-specific CD8 + lymphocytes. Furthermore, we found a positivecorrelation between both factors in the patients with HAM/TSP (P = 0.044) but not in the AC (P = 0.508). These findings suggest that the high number of HTLV-I-specific lymphocytes may result from the increased proviral load in HAM/TSP patients. (C) 2000 Published by Elsevier Science B.V. All rightsreserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 14:06:21