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Titolo:
Human immunodeficiency virus type-1 Nef protein induces blood-brain barrier disruption in the rat: role of matrix metalloproteinase-9
Autore:
Sporer, B; Koedel, U; Paul, R; Kohleisen, B; Erfle, V; Fontana, A; Pfister, HW;
Indirizzi:
Univ Munich, Klinikum Grosshadern, Dept Neurol, D-81377 Munich, Germany Univ Munich Munich Germany D-81377 Dept Neurol, D-81377 Munich, Germany GSF, Res Ctr Environm & Hlth, Inst Mol Virol, Neuherberg, Germany GSF Neuherberg Germany ronm & Hlth, Inst Mol Virol, Neuherberg, Germany Univ Zurich, Inst Clin Immunol, Zurich, Switzerland Univ Zurich Zurich Switzerland , Inst Clin Immunol, Zurich, Switzerland
Titolo Testata:
JOURNAL OF NEUROIMMUNOLOGY
fascicolo: 2, volume: 102, anno: 2000,
pagine: 125 - 130
SICI:
0165-5728(20000124)102:2<125:HIVTNP>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; NECROSIS-FACTOR-ALPHA; CELL-LINES; HIV-1 INFECTION; ENDOTHELIAL-CELLS; IV COLLAGENASE; T-CELLS; ASTROCYTES; TUMOR; LOCALIZATION;
Keywords:
HIV-1; Nef; brain; rat; matrix metalloproteinase; MMP; batimastat; blood-brain barrier;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Pfister, HW Univ Munich, Klinikum Grosshadern, Dept Neurol, Marchioninistr15, D-81377Munich, Germany Univ Munich Marchioninistr 15 Munich Germany D-81377 Germany
Citazione:
B. Sporer et al., "Human immunodeficiency virus type-1 Nef protein induces blood-brain barrier disruption in the rat: role of matrix metalloproteinase-9", J NEUROIMM, 102(2), 2000, pp. 125-130

Abstract

We recently showed that MMP-9 activity was detectable in the cerebrospinalfluid (CSF) of about half of neurologically symptomatic HIV-infected patients. Using an experimental animal model, we detected MMP-9 activity in CSF samples from rats that had been injected intracisternally with recombinant HIV-1 Nef protein, but not after injection of heat-treated Nef, gp120, gp160 or PBS. Nef also induced a breaching of the blood-brain barrier (BBB), which could be inhibited by pretreatment with the matrix metalloproteinase (MMP) inhibitor batimastat. In vitro Nef only slightly induced MMP-9 activityin freshly isolated human peripheral blood mononuclear cells and in the murine macrophage cell line RAW 264.7, but not in endothelial, neuronal or astroglial cell lines. Taken together, our findings indicate that HIV-1 Nef protein can induce BBB disruption in the rat - presumably via MMP induction. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/05/20 alle ore 15:17:56