Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Virus-like particles assemble in plants and bacteria expressing the coat protein gene of Indian peanut clump virus
Autore:
Bragard, C; Duncan, GH; Wesley, SV; Naidu, RA; Mayo, MA;
Indirizzi:
Catholic Univ Louvain, Unite Phytopathol, B-3000 Louvain, Belgium CatholicUniv Louvain Louvain Belgium B-3000 ol, B-3000 Louvain, Belgium Scottish Crop Res Inst, Dundee DD2 5DA, Scotland Scottish Crop Res Inst Dundee Scotland DD2 5DA Dundee DD2 5DA, Scotland Int Crops Res Inst Semi Arid Trop, Patancheru 502324, Andhra Pradesh, India Int Crops Res Inst Semi Arid Trop Patancheru Andhra Pradesh India 502324
Titolo Testata:
JOURNAL OF GENERAL VIROLOGY
, volume: 81, anno: 2000,
parte:, 1
pagine: 267 - 272
SICI:
0022-1317(200001)81:<267:VPAIPA>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; MOSAIC-VIRUS; TRANSGENIC PLANTS; POTATO; RESISTANCE; SEQUENCE; TOBACCO; RNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Bragard, C Catholic Univ Louvain, Unite Phytopathol, B-3000 Louvain, Belgium Catholic Univ Louvain Louvain Belgium B-3000 Louvain, Belgium
Citazione:
C. Bragard et al., "Virus-like particles assemble in plants and bacteria expressing the coat protein gene of Indian peanut clump virus", J GEN VIROL, 81, 2000, pp. 267-272

Abstract

cDNA copies of the coat protein (CP) gene of Indian peanut clump virus (IPCV)-H were introduced into cells of Nicotiana benthamiana or Escherichia coli by transformation with vectors based on pROKII or pET respectively. In both plant and bacterial cells, IPCV CP was expressed and assembled to form virus-like particles (VLP). In plant extracts, the smallest preponderant particle length was about 50 nm, Other abundant lengths were about 85 and about 120 nm, The commonest VLP length in bacterial extracts was about 30 nm. Many of the longer VLP appeared to comprise aggregates of shorter particles. The lengths of the supposed 'monomer' VLP corresponded approximately to those expected for encapsidated CP gene transcript RNA. Immunocapture RT-PCR, using primers designed to amplify the CP gene, confirmed that the VLP contained RNA encoding IPCV-H CP. The results show that encapsidation does notrequire the presence of the 5'-terminal untranslated sequence of the virusRNA and suggest that if there is an 'origin of assembly' motif or sequence, it lies within the CP gene. When transgenic plants expressing IPCV-H CP were inoculated with IPCV-L, a strain that is serologically distinct from IPCV-H, the virus particles that accumulated contained both types of CP.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/08/20 alle ore 20:16:28