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Titolo:
Extended tropism of an adenoviral vector does not circumvent the maturation-dependent transducibility of mouse skeletal muscle
Autore:
van Deutekom, JCT; Cao, BH; Pruchnic, R; Wickham, TJ; Kovesdi, I; Huard, J;
Indirizzi:
Childrens Hosp Pittsburgh, Musculoskeletal Res Ctr, Dept Orthopaed Surg, Pittsburgh, PA 15213 USA Childrens Hosp Pittsburgh Pittsburgh PA USA 15213 ittsburgh, PA 15213 USA GenVec Inc, Rockville, MD 20854 USA GenVec Inc Rockville MD USA 20854GenVec Inc, Rockville, MD 20854 USA Univ Pittsburgh, Dept Mol Genet & Biochem, Pittsburgh, PA 15213 USA Univ Pittsburgh Pittsburgh PA USA 15213 Biochem, Pittsburgh, PA 15213 USA
Titolo Testata:
JOURNAL OF GENE MEDICINE
fascicolo: 6, volume: 1, anno: 1999,
pagine: 393 - 399
SICI:
1099-498X(199911/12)1:6<393:ETOAAV>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
FULL-LENGTH DYSTROPHIN; MEDIATED GENE-TRANSFER; VIRAL GENES; AIRWAY EPITHELIA; SUBGROUP-C; DELIVERY; CELLS; EXPRESSION; RECEPTORS; RECOMBINANTS;
Keywords:
muscle maturation; extended tropism adenovirus (AdPK); adenoviral receptors; extracellular matrix; dy/dy mouse; mdx mouse;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Huard, J Childrens Hosp, Growth & Dev Lab, Dept Orthopaed Surg & Mol Genet& Biochem, 4151 Rangos Res Ctr,3705 5th Ave, Pittsburgh, PA 15213 USA Childrens Hosp 4151 Rangos Res Ctr,3705 5th Ave Pittsburgh PA USA 15213
Citazione:
J.C.T. van Deutekom et al., "Extended tropism of an adenoviral vector does not circumvent the maturation-dependent transducibility of mouse skeletal muscle", J GENE MED, 1(6), 1999, pp. 393-399

Abstract

Background Efficient adenoviral gene delivery to mature skeletal muscle has been hindered by different factors. The low levels of adenoviral attachment receptor (CAR) that have been reported in this tissue may be a limiting factor. Therefore, adenoviral transduction of mature muscle may be improvedby extending the tropism of the adenoviral vectors to attachment receptorsthat are highly expressed in mature myofibers. In this study, we have investigated whether an extended tropism adenoviral vector which additionally attaches to the broadly expressed heparan-containing receptors (AdPK) can bypass the maturation-dependent adenoviral transducibility of mouse skeletal muscle. Methods The adenoviral vector AdPK carrying the LacZ gene was evaluated asa gene delivery vehicle in mouse skeletal muscle at different maturities in vitro and in vivo. The viral transduction efficiencies were determined byhistochemical and ONPG analysis of the beta-galactosidase activity level. Results Higher transduction efficiencies were detected in immature muscle from normal mice, and in mature muscle from merosin-deficient dy/dy mice (carrying myofibers with an impaired extracellular matrix) and dystrophin-deficient mdx mice (showing a high level of myoblast activity) when compared to mature muscle from normal mice. Conclusions Despite the enhanced attachment characteristics, the extended tropism adenoviral vector is, similarly to the wild-type adenoviral vector in previous studies, still hindered by both a protective extracellular matrix and the diminished myoblast-mediation in mature muscle. Copyright (C) 1999 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 20:09:12