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Titolo:
Development of a protocol for the automated analysis of amino acids in brain tissue samples and microdialysates
Autore:
Shah, AJ; de Biasi, V; Taylor, SG; Roberts, C; Hemmati, P; Munton, R; West, A; Routledge, C; Camilleri, P;
Indirizzi:
SmithKline Beecham Pharmaceut, Harlow CM19 5AW, Essex, England SmithKline Beecham Pharmaceut Harlow Essex England CM19 5AW ssex, England
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 735, anno: 1999,
pagine: 133 - 140
SICI:
1387-2273(199912)735:2<133:DOAPFT>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; CENTRAL NERVOUS-SYSTEM; ELECTROCHEMICAL DETECTION; RAT-BRAIN; NEUROTRANSMITTERS; DERIVATIZATION; NAPHTHALENE-2,3-DICARBOXALDEHYDE; DEMENTIA; REAGENT; PHASE;
Keywords:
amino acids;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Shah, AJ SmithKline Beecham Pharmaceut, New Frontier Sci Pk,3rd Ave, Harlow CM19 5AW, Essex, England SmithKline Beecham Pharmaceut New Frontier Sci Pk,3rd Ave Harlow Essex England CM19 5AW
Citazione:
A.J. Shah et al., "Development of a protocol for the automated analysis of amino acids in brain tissue samples and microdialysates", J CHROMAT B, 735(2), 1999, pp. 133-140

Abstract

An automated precolumn derivatisation method has been developed for the measurement of fourteen amino acids in brain tissue and microdialysate samples. The method involves labelling amino acids with naphthalene-2,3-dicarboxaldehyde (NDA) in the presence of cyanide (CN-). The resulting highly stableN-substituted 1-cyanobenz[f]isoindole (CBI) derivatives were separated using a binary gradient elution profile and detected fluorometrically. The order of elution of the derivatised amino acids was confirmed by using liquid chromatography with fluorescence and mass spectrometric detection in tandem. Linear calibration plots were obtained for all amino acids in the range studied (0.2-12.5 mu M). The limit of detection for CBI derivatives of aminoacids was in the range 5-20 fmol (S/N=2) using a 5 mu l injection volume. The method has been used for the measurement of amino acids in microdialysates from rat brain and tissue homogenates from different regions of mouse brain. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 23:00:54