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Titolo:
Direct injection of physiological fluids in micellar liquid chromatography
Autore:
Alvarez-Coque, MCG; Broch, SC;
Indirizzi:
Univ Valencia, Fac Quim, Dept Quim Analit, E-46100 Burjassot, Spain Univ Valencia Burjassot Spain E-46100 m Analit, E-46100 Burjassot, Spain
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1-2, volume: 736, anno: 1999,
pagine: 1 - 18
SICI:
1387-2273(199912)736:1-2<1:DIOPFI>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
SODIUM DODECYL-SULFATE; DIRECT SERUM INJECTION; UNTREATED BODY-FLUIDS; MOBILE PHASES; HUMAN-PLASMA; BIOLOGICAL-FLUIDS; HPLC COLUMN; ELECTROKINETIC CHROMATOGRAPHY; FLUORESCENCE DETECTION; FLUOROMETRIC DETECTION;
Keywords:
reviews; micellar liquid chromatography; physiological samples; direct injection;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
87
Recensione:
Indirizzi per estratti:
Indirizzo: Alvarez-Coque, MCG Univ Valencia, Fac Quim, Dept Quim Analit, Doctor Moliner 50, E-46100 Burjassot, Spain Univ Valencia Doctor Moliner 50 Burjassot Spain E-46100
Citazione:
M.C.G. Alvarez-Coque e S.C. Broch, "Direct injection of physiological fluids in micellar liquid chromatography", J CHROMAT B, 736(1-2), 1999, pp. 1-18

Abstract

Micellar liquid chromatography (MLC), which uses mobile phases of surfactants above the critical micellar concentration, provides a solution to the direct injection of physiological samples by solubilizing the protein components, and coating the analytical column with surfactant monomers to avoid clogging. A review showing the advantages and limitations of this technique over other chromatographic techniques used in drug analysis, working protocols, and examples of application is presented. The possibility of direct sample introduction simplifies and greatly expedites the treatments with reduced cost, improving the accuracy of the procedures. Surfactant monomers andmicelles appear to displace drugs bound to proteins, releasing them for partitioning to the stationary phase. The versatility of MLC encompasses the wide range of drug classes normally monitored, such as analgesics, anticancer drugs, antidepressants, bacteriostats, beta-blockers, bronchodilators, catecholamines, diuretics and steroids, among others. Analytical procedures have been developed in urine, plasma, serum and cow milk samples. Most of them utilize sodium dodecyl sulphate as surfactant and a C-18 column. UV detection is usual, but enhanced detection has been reported by measuring the absorbance in the visible region of drug derivatives formed precolumn, and with a variety of other techniques, such as fluorimetry, amperometry, inductively coupled plasma-mass spectrometry and immunoassay. Column-switching with on-line surfactant-mediated sample clean-up is shown as an attractive enrichment technique, which expands the practical use of MLC beyond the singular dimensional chromatographic process. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 11:49:26