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Titolo:
Expression of a single fair of desmosomal glycoproteins readers the corneal epithelium unique amongst stratified epithelia
Autore:
Messent, AJ; Blissett, MJ; Smith, GL; North, AJ; Magee, A; Foreman, D; Garrod, DR; Boulton, M;
Indirizzi:
Cardiff Univ, Dept Optometry & Vis Sci, Cell & Mol Biol Unit, Cardiff CF1 3XF, S Glam, Wales Cardiff Univ Cardiff S Glam Wales CF1 3XF Cardiff CF1 3XF, S Glam, Wales Univ Manchester, Sch Biol Sci, Dept Ophthalmol, Manchester, Lancs, EnglandUniv Manchester Manchester Lancs England mol, Manchester, Lancs, England Univ Manchester, Sch Biol Sci, Epithelial Morphogenesis Reg Grp, Manchester, Lancs, England Univ Manchester Manchester Lancs England Grp, Manchester, Lancs, England Natl Inst Med Res, Div Membrane Biol, London NW7 1AA, England Natl Inst Med Res London England NW7 1AA e Biol, London NW7 1AA, England
Titolo Testata:
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
fascicolo: 1, volume: 41, anno: 2000,
pagine: 8 - 15
SICI:
0146-0404(200001)41:1<8:EOASFO>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
PEMPHIGUS-VULGARIS ANTIGEN; HUMAN EPIDERMIS; PLAKOGLOBIN; CADHERINS; ISOFORMS; DESMOPLAKIN; PATTERNS; PROTEIN; FAMILY; MOUSE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Boulton, M Cardiff Univ, Dept Optometry & Vis Sci, Cell & Mol Biol Unit, POB 905,Redwood Bldg, Cardiff CF1 3XF, S Glam, Wales Cardiff Univ POB 905,Redwood Bldg Cardiff S Glam Wales CF1 3XF
Citazione:
A.J. Messent et al., "Expression of a single fair of desmosomal glycoproteins readers the corneal epithelium unique amongst stratified epithelia", INV OPHTH V, 41(1), 2000, pp. 8-15

Abstract

PURPOSE. To determine desmosomal glycoprotein isoform expression in bovinecorneal, limbal, and conjunctival epithelium and desmosomal profile and distribution during corneal re-epithelialization. METHODS. Immunofluorescence (IF) for desmosomal components on cryostat sections of fresh epithelia was supported by immunoblot analysis of tissue lysates. Wounded corneas maintained in organ culture were examined by IF at times up to full re-epithelialization (96 hours). RESULTS. Immunofluorescence for desmoplakin confirmed desmosome presence throughout all three epithelia. Plakoglobin was also ubiquitous. Of the desmosomal glycoproteins, desmocollin 7 (Dsc2) and desmoglein 2 (Dsg2) were expressed throughout, but Dsc3 and Dsg3 were confined to the limbus and conjunctiva, and Dsc1 and Dsg1 were absent. Dsc2 and Dsg3 Ifs were stronger in superficial layers, but Dsc3 and Dsg3 were stronger basally, fading suprabasally. Glycoprotein expression in cornea and conjunctiva was confirmed by immunoblot analysis. No change in glycoprotein expression occurred during re-epithelialization. CONCLUSIONS. Uniquely among stratified epithelia, cornea expresses only a single pair of desmosomal glycoproteins, Dsc2 and Dsg2. Expression of Dsc3 and Dsg3 in limbus and conjunctiva coincides with their association with cell proliferation in other epithelia, bur corneal epithelial cells did not express Dsc3 or Dsg3 during re-epithelialization. Absence of Dsc1 and Dsg1 correlates with lack of keratinization in ocular epithelia. These expressionpatterns may have significance for the specific properties and differentiation patterns of the epithelia. Presence of desmosomes throughout re-epithelialization raises the question of how migrating cells mutually re-position.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 02:36:08