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Titolo:
HIV-1 subtype C syncytium- and non-syncytium-Ethiopian patients with AIDS
Autore:
Abebe, A; Demissie, D; Goudsmit, J; Brouwer, M; Kuiken, CL; Pollakis, G; Schuitemaker, H; Fontanet, AL; de Wit, TFR;
Indirizzi:
Ethiopian Hlth & Nutr Res Inst, Ethiopian Netherlands AIDS Res Project, Addis Ababa, Ethiopia Ethiopian Hlth & Nutr Res Inst Addis Ababa Ethiopia dis Ababa, Ethiopia
Titolo Testata:
ETHIOPIAN MEDICAL JOURNAL
, volume: 37, anno: 1999, supplemento:, 1
pagine: 79 - 85
SICI:
0014-1755(199910)37:<79:HSCSAN>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; BIOLOGICAL PHENOTYPE; GENOTYPIC CHARACTERISTICS; SEQUENCE-ANALYSIS; CORECEPTOR USAGE; TYPE-1 INFECTION; VIRAL PHENOTYPE; ADDIS-ABABA; T-CELL; INDIVIDUALS;
Keywords:
HIV-1; C-subtype; AIDS; SI; NSI; coreceptor; Ethiopia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Abebe, A Ethiopian Hlth & Nutr Res Inst, Ethiopian Netherlands AIDS Res Project, POB 1242, Addis Ababa, Ethiopia Ethiopian Hlth & Nutr Res Inst POB 1242 Addis Ababa Ethiopia ia
Citazione:
A. Abebe et al., "HIV-1 subtype C syncytium- and non-syncytium-Ethiopian patients with AIDS", ETHIOP MED, 37, 1999, pp. 79-85

Abstract

Objective: To assess syncytium-inducing (SI) and non-syncytium-inducing (NS1) frequencies, coreceptor usage and gp120 V3 sequences of HIV-1 isolates from Ethiopian AIDS patients. Patients: Cross-sectional study on 48 hospitalized AIDS patients (CD4 T cells < 200 x 10(6) cell/l) with stage III or IV of the WHO staging system for HIV-1 infection and disease. Methods: Peripheral blood mononuclear cells (PBMC) from all 48 patients-were tested by MT-2 assay to determine SI/NSI phenotypes. Lymphocyte subsets were enumerated using Coulter counting and FACScan analysis. Viral load determination used a nucleic acid sequence-based amplification assay (NASBA). Coreceptor:usage of HIV-1 biological clones was measured using U87 CD4/chemokine receptor transfectants and phytohemagglutinin-stimulated PBMC of healthy donors with wildtype CCR5 and homozygous mutation CCR5 Delta 32 (a 32 base-pair deletion in CCR5). Reverse transcriptase polymerase chain reactionsequencing was performed on the third variable region (V3) of the HIV-1 gene gp120. Sequence alignments were done manually; phylogenetic analyses used PHYLIP software packages. Results: SI viruses were detected for 3/48 (6%) AIDS patients only. Lower mean absolute CD4 counts were determined in patients with SI virus comparedwith NSI (P = 0.04), but no differences in viral load were observed. All patients were found to be infected with HIV-1 subtype C, based on V3 sequencing. NSI biological clones used CCR5 as coreceptor; SI biological clones used CXCR4 and/or CCR5 and/or ;CCR3. Conclusions: Ethiopian patients with HIV-1 C-subtype AIDS harbour a remarkably low frequency of SI phenotype viruses. Coreceptor usage of these viruses correlates with their biological phenotypes. (C) 1999 Lippincott Williams & Wilkins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 15:05:24