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Titolo:
Mutations in the Rho1 small GTPase disrupt morphogenesis and segmentation during early Drosophila development
Autore:
Magie, CR; Meyer, MR; Gorsuch, MS; Parkhurst, SM;
Indirizzi:
Fred Hutchinson Canc Res Ctr, Div Basic Sci, Seattle, WA 98109 USA Fred Hutchinson Canc Res Ctr Seattle WA USA 98109 , Seattle, WA 98109 USA Fred Hutchinson Canc Res Ctr, Pprogram Dev Biol, Seattle, WA 98109 USA Fred Hutchinson Canc Res Ctr Seattle WA USA 98109 , Seattle, WA 98109 USA Univ Washington, Dept Zool, Seattle, WA 98195 USA Univ Washington SeattleWA USA 98195 on, Dept Zool, Seattle, WA 98195 USA
Titolo Testata:
DEVELOPMENT
fascicolo: 23, volume: 126, anno: 1999,
pagine: 5353 - 5364
SICI:
0950-1991(199912)126:23<5353:MITRSG>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-SHAPE CHANGES; AMINO-TERMINAL KINASE; ACTIN STRESS FIBERS; BINDING PROTEIN-RHO; INSERTIONAL MUTAGENESIS; SIGNAL-TRANSDUCTION; SHEET MOVEMENT; GENE; RAC; FAMILY;
Keywords:
Rho GTPase; Drosophila; morphogenesis; cytoskeleton; dorsal closure; head involution; concertina; cappuccino;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
72
Recensione:
Indirizzi per estratti:
Indirizzo: Parkhurst, SM Fred Hutchinson Canc Res Ctr, Div Basic Sci, A1-162,1100 Fairview Ave N, Seattle, WA 98109 USA Fred Hutchinson Canc Res Ctr A1-162,1100Fairview Ave N Seattle WA USA 98109
Citazione:
C.R. Magie et al., "Mutations in the Rho1 small GTPase disrupt morphogenesis and segmentation during early Drosophila development", DEVELOPMENT, 126(23), 1999, pp. 5353-5364

Abstract

Rho GTPases play an important role in diverse biological processes such asactin cytoskeleton organization, gene transcription, cell cycle progression and adhesion. They are required during early Drosophila development for proper execution of morphogenetic movements of individual cells and groups of cells important for the formation of the embryonic body plan. We isolatedloss-of-function mutations in the Drosophila Rho1 (Rho1) gene during a genetic screen for maternal-effect mutations, allowing us to investigate the specific roles Rho1 plays in the contest of the developing organism. Here wereport that Rho1 is required for many early events: loss of Rho1 function results in both maternal and embryonic phenotypes. Embryos homozygous for the Rho1 mutation exhibit a characteristic zygotic phenotype, which includessevere defects in head involution and imperfect dorsal closure. Two phenotypes are associated with reduction of maternal Rho1 activity: the actin cytoskeleton is disrupted in egg chambers, especially in the ring canals and embryos display patterning defects as a result of improper maintenance of segmentation gene expression. Despite showing imperfect dorsal closure, Rho1 does not activate downstream genes or interact genetically with members of the JNK signaling pathway, used by its relatives dRac and dCdc42 for properdorsal closure. Consistent with its roles in regulating actin cytoskeletalorganization, we find that Rho1 interacts genetically and physically with the Drosophila formin homologue, cappuccino. We also show that Rho1 interacts both genetically and physically with concertina, a G alpha protein involved in cell shape changes during gastrulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 09:57:42