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Titolo:
Serum amyloid A protein generates pre beta 1 high-density lipoprotein fromalpha-migrating high-density lipoprotein
Autore:
Miida, T; Yamada, T; Yamadera, T; Ozaki, K; Inano, K; Okada, M;
Indirizzi:
Niigata Univ, Sch Med, Dept Lab Med, Niigata 9518510, Japan Niigata Univ Niigata Japan 9518510 Dept Lab Med, Niigata 9518510, Japan Niigata Univ, Sch Med, Dept Internal Med 1, Niigata 9518510, Japan NiigataUniv Niigata Japan 9518510 nternal Med 1, Niigata 9518510, Japan Jichi Med Sch, Dept Clin Pathol, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 3290498 Tochigi 3290498, Japan Niigata Univ Hosp, Div Pharm, Niigata 9518520, Japan Niigata Univ Hosp Niigata Japan 9518520 iv Pharm, Niigata 9518520, Japan
Titolo Testata:
BIOCHEMISTRY
fascicolo: 51, volume: 38, anno: 1999,
pagine: 16958 - 16962
SICI:
0006-2960(199912)38:51<16958:SAAPGP>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHOSPHOLIPID TRANSFER PROTEIN; APO-A-I; CHOLESTEROL EFFLUX; HEPATIC LIPASE; HUMAN PLASMA; APOLIPOPROTEIN; PARTICLES; HDL; SAA; TRANSGENES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Miida, T Niigata Univ, Sch Med, Dept Lab Med, Asahimachi 1-757, Niigata 9518510, Japan Niigata Univ Asahimachi 1-757 Niigata Japan 9518510 18510, Japan
Citazione:
T. Miida et al., "Serum amyloid A protein generates pre beta 1 high-density lipoprotein fromalpha-migrating high-density lipoprotein", BIOCHEM, 38(51), 1999, pp. 16958-16962

Abstract

Serum amyloid A protein (SAA), an acute-phase reactant in reactive amyloidosis, has high affinity for high-density Lipoprotein (HDL). When SAA is added to HDL, SAA displaces apolipoprotein A-I (apoA-I) and phospholipid from the HDL particles. These dissociated components may form pre beta 1-HDL because free apoA-I can associate with phospholipid to become a lipoprotein having pre beta mobility. To determine whether SAA generates pre beta 1-HDL from alpha-migrating HDL, we investigated the effects of recombinant SAA on HDL subfraction concentration using nondenaturing two-dimensional gradient gel electrophoresis. When we added SAA (0.5 mg/mL) to plasma, the pre beta 1-HDL concentration increased by 164% (from 4.7% +/- 1.3% to 12.4% +/- 3.2%of apoA-I, p < 0.005), The increase in pre beta 1-HDL was proportional to the dose of SAA, When we added SAA to a column of Sepharose beads coupled to the isolated HDL (alpha-migrating HDL), pre beta 1-HDL was dissociated from the column together with the SAA-associated HDL. In summary, we demonstrate that SAA generates pre beta 1-HDL from alpha-migrating HDL. We speculate that SAA-mediated HDL remodeling may take place in inflammation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 23:03:58