Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
EVALUATION OF 2 HOMOGENEOUS METHODS FOR MEASURING HIGH-DENSITY-LIPOPROTEIN CHOLESTEROL
Autore:
HUANG YC; KAO JT; TSAI KS;
Indirizzi:
NATL TAIWAN UNIV HOSP,COLL MED,DEPT LAB MED,7 CHUNG SHAN S RD TAIPEI 10016 TAIWAN NATL TAIWAN UNIV HOSP,COLL MED,DEPT LAB MED TAIPEI 10016 TAIWAN NATL TAIWAN UNIV,COLL MED,SCH MED TECHNOL TAIPEI 10016 TAIWAN MUNICIPAL HO PIN HOSP,DEPT LAB MED TAIPEI TAIWAN
Titolo Testata:
Clinical chemistry
fascicolo: 6, volume: 43, anno: 1997,
parte:, 1
pagine: 1048 - 1055
SICI:
0009-9147(1997)43:6<1048:EO2HMF>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
CORONARY HEART-DISEASE; PRECIPITATION; FRAMINGHAM; SERUM;
Keywords:
METHODS COMPARISON; TRIGLYCERIDES; ATHEROSCLEROSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
Y.C. Huang et al., "EVALUATION OF 2 HOMOGENEOUS METHODS FOR MEASURING HIGH-DENSITY-LIPOPROTEIN CHOLESTEROL", Clinical chemistry, 43(6), 1997, pp. 1048-1055

Abstract

We evaluated the performance of two homogeneous assays for quantifying HDL cholesterol (HDL-C) and compared them with the phosphotungstic acid (PTA)/MgCl2 assay. Both homogeneous HDL-C assays were precise, having a within-run CV of <1.20% and a between-run CV of <4.07%. The HDL-C values (y) measured by the two homogeneous methods correlated well with those by the PTA/MgCl2 method (x): y = 1.00x + 64.98 mg/L, r = 0.987, S-y/x = 27.99 mg/L (n = 152) for the polyethylene glycol-modified enzymes/alpha-cyclodextrin sulfate (PEGME) assay (Kyowa), and y = 0.84x + 106.51 mg/L, r = 0.984, S-y/x = 26.10 mg/L (n = 152) for the polyanion-polymer/detergent (PPD) assay (Daiichi). The specificity of the PEGME method seemed better than that of the PPD method, as the PPD method was markedly interfered with by supplemental LDL-C. Addition of 20 g/L triglycerides produced a negative error of similar to 18% in both homogeneous assays. Bilirubin and hemoglobin had little influence on the PEGME method; hemoglobin had little effect on the PPD method. Bilirubin, however, markedly decreased the readings by the PPD method. We found the PEGME assay superior to the PPD assay for routine HDL-C testing, because the PPD assay is relatively inaccurate and not specific.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 02:26:45