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Titolo:
Altered endothelin receptor subtype expression in hepatic injury after ischemia/reperfusion
Autore:
Yokoyama, Y; Baveja, R; Sonin, N; Nakanishi, K; Zhang, JX; Clemens, MG;
Indirizzi:
Univ N Carolina, Dept Biol, Charlotte, NC 28223 USA Univ N Carolina Charlotte NC USA 28223 Dept Biol, Charlotte, NC 28223 USA
Titolo Testata:
SHOCK
fascicolo: 1, volume: 13, anno: 2000,
pagine: 72 - 78
SICI:
1073-2322(200001)13:1<72:AERSEI>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
ISCHEMIA-REPERFUSION INJURY; ORTHOTOPIC LIVER-TRANSPLANTATION; RAT-LIVER; MICROVASCULAR INJURY; NITRIC-OXIDE; MICROCIRCULATORY FAILURE; ANTAGONIST TAK-044; NEUTROPHILS; CONTRACTION; ACTIVATION;
Keywords:
endothelin A receptor; endothelin B receptor; IRL 1620; BQ 610; nitric oxide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Clemens, MG Univ N Carolina, Dept Biol, 9201 Univ City Blvd, Charlotte, NC28223 USA Univ N Carolina 9201 Univ City Blvd Charlotte NC USA 28223 USA
Citazione:
Y. Yokoyama et al., "Altered endothelin receptor subtype expression in hepatic injury after ischemia/reperfusion", SHOCK, 13(1), 2000, pp. 72-78

Abstract

This study was performed to determine whether ischemia/reperfusion (I/R) injury in rat liver results in alterations in endothelin receptor expression. Hepatic ischemia was produced in rats for 60 min followed by 6 or 24 h reperfusion. Portal inflow pressure was increased (7.38 +/- 0.60 mmHg) at 24 hours after reperfusion, Serum ALT increased significantly at both 6 and 24h (6 h; 258.3 +/- 74.3, 24 h; 243.1 +/- 74.8 IU/L). Portal vascular response to an endothelin-B receptor agonist (IRL 1620) was significantly increased in the I/R livers compared to control and this was potentiated by L-NAME, IRL 1620 also caused LDH release from I/R livers but not controls. LDH release after IRL 1620 in I/R livers correlated with increased portal pressure response. To determine whether the altered response might be the result of altered endothelin receptor expression, livers were harvested after reperfusion and total endothelin binding sites were determined by competitive binding with ET-1. Proportion of endothelin receptor subtypes (ETA/ETB) was determined using the ETA antagonist BQ-610 (1 mu M) and ETB agonist IRL-1620(100 nM). There were no significant changes in Kd but Bmax for endothelin-1 was decreased in I/R group especially non-ischemic lobe at 24 h. ETA receptors were significantly decreased whereas ETB receptors were increased. These changes were more pronounced at 24 h after reperfusion than at 6 h. Interestingly, the changes in ET receptors was observed identically both in ischemic and non-ischemic lobes (ischemic lobe ETA 41.9%, ETB 51%, non-ischemic robe ETA 38.8%, ETB 49.5%). These results indicate that the major functional endothelin receptor subtype upregulated in I/R is the ETB receptor andthat this upregulation may contribute to microvascular dysregulation and hepatic injury.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 10:31:10