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Titolo:
Nonionic surfactant-mediated affinity cloud-point extraction of vancomycin
Autore:
Lee, CK; Su, WD;
Indirizzi:
Natl Taiwan Inst Technol, Dept Chem Engn, Taipei 106, Taiwan Natl Taiwan Inst Technol Taipei Taiwan 106 Chem Engn, Taipei 106, Taiwan
Titolo Testata:
SEPARATION SCIENCE AND TECHNOLOGY
fascicolo: 16, volume: 34, anno: 1999,
pagine: 3267 - 3277
SICI:
0149-6395(1999)34:16<3267:NSACEO>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
AQUEOUS 2-PHASE SYSTEMS; GLYCOPEPTIDE ANTIBIOTICS; CHROMATOGRAPHY; MACROLIGAND;
Keywords:
cloud-point extraction; temperature-induced phase separation; C10E4; vancomycin; CMC of vancomycin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Lee, CK Natl Taiwan Inst Technol, Dept Chem Engn, Taipei 106, Taiwan Natl Taiwan Inst Technol Taipei Taiwan 106 n, Taipei 106, Taiwan
Citazione:
C.K. Lee e W.D. Su, "Nonionic surfactant-mediated affinity cloud-point extraction of vancomycin", SEP SCI TEC, 34(16), 1999, pp. 3267-3277

Abstract

The partitioning behaviors of vancomycin in a temperature-induced nonionicsurfactant two-phase system were studied. N-Decyltetra(ethylene oxide) (C10E4) was employed as the nonionic surfactant. Vancomycin had a preference for the micelle-rich top phase under most experimental conditions. However, at pH 4 vancomycin preferred to stay in the micelle-poor bottom phase. An affinity cosurfactant, D-alanyl-D-alanine modified cholesterol, was employedin the extraction system to increase the partition coefficient of vancomycin. The I,partition coefficient increased significantly from 0.87 to 15.98 when an affinity cosurfactant was employed. Vancomycin could form a micelle, and its critical micelle concentration (CMC) was determined by the methylorange binding technique and the surface tension method. The micelle formation of vancomycin was considered to induce the lower partition coefficientand a significant increase of the partition coefficient in the presence ofthe affinity cosurfactant at pH 4. Direct recovery of vancomycin From fermentation broth using affinity extraction was also demonstrated.

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Documento generato il 03/12/20 alle ore 15:02:58