Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
UNENCAPSIDATEDD DOUBLE-STRANDED-RNA ASSOCIATED WITH MEMBRANE-VESICLESIN ISOLATES OF ALTERNARIA-SOLANI
Autore:
ZABALGOGEAZCOA I; PETRUNAK D; CHRIST BJ; GILDOW FE;
Indirizzi:
PENN STATE UNIV,DEPT PLANT PATHOL UNIVERSITY PK PA 16802 CSIC,INST RECURSOS NAT & AGROBIOL E-37071 SALAMANCA SPAIN
Titolo Testata:
Mycological research
, volume: 101, anno: 1997,
parte:, 5
pagine: 604 - 608
SICI:
0953-7562(1997)101:<604:UDAWM>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHIA-PARASITICA; HYPOVIRULENT STRAINS; GENETIC ELEMENTS; RIBONUCLEIC-ACID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
I. Zabalgogeazcoa et al., "UNENCAPSIDATEDD DOUBLE-STRANDED-RNA ASSOCIATED WITH MEMBRANE-VESICLESIN ISOLATES OF ALTERNARIA-SOLANI", Mycological research, 101, 1997, pp. 604-608

Abstract

Double-stranded RNA (dsRNA) elements of 8.3 and 5.5 kbp were detectedin three of Eve geographically distinct isolates of Alternaria solani. Variation with respect to the number of dsRNA segments was detected among isolates of the fungus. No virus-like particles were purified from the mycelium, or observed in ultra-thin sections of hyphae. Utilizing differential centrifugation or density gradient centrifugation for subcellular fractionation, dsRNA was found associated specifically with fractions containing 80-100 nm spherical membrane vesicles. Similar vesicles were observed in cytoplasm of hyphae by electron microscopy. Treatment of dsRNA-containing fractions with ribonuclease A did not degrade the dsRNA, suggesting that it is located inside the vesicles. There was no correlation between the radial growth of the isolates in laboratory media and the presence or absence or dsRNA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 02:24:49