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Titolo:
Purification and properties of a major isoform of beta-1,3-glucanase from pearl millet seedlings
Autore:
Kini, KR; Vasanthi, NS; Umesh-Kumar, S; Shetty, HS;
Indirizzi:
Univ Mysore, Downy Mildew Res Lab, Dept Studies Appl Bot & Biotechnol, Mysore 570006, Karnataka, India Univ Mysore Mysore Karnataka India 570006 Mysore 570006, Karnataka, India Cent Food Technol Res Inst, Mol Biol Unit, Dept Microbiol & Bioengn, Mysore 570013, Karnataka, India Cent Food Technol Res Inst Mysore Karnataka India 570013 Karnataka, India
Titolo Testata:
PLANT SCIENCE
fascicolo: 2, volume: 150, anno: 2000,
pagine: 139 - 145
SICI:
0168-9452(20000128)150:2<139:PAPOAM>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
PATHOGENESIS-RELATED PROTEINS; BARLEY HORDEUM-VULGARE; PHYTOPHTHORA-CAPSICI; BIOLOGICAL FUNCTION; PEPPER STEMS; CHITINASE; INFECTION; INDUCTION; LEAVES; 1,3-BETA-GLUCANASE;
Keywords:
beta-1,3-glucanase; pearl miller; downy mildew; host-pathogen interaction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Shetty, HS Univ Mysore, Downy Mildew Res Lab, Dept Studies Appl Bot & Biotechnol, Mysore 570006, Karnataka, India Univ Mysore Mysore Karnataka India570006 06, Karnataka, India
Citazione:
K.R. Kini et al., "Purification and properties of a major isoform of beta-1,3-glucanase from pearl millet seedlings", PLANT SCI, 150(2), 2000, pp. 139-145

Abstract

A major isoform of beta-1,3-glucanase from pearl miller seedlings was purified following ammonium sulfate precipitation, ion-exchange chromatography and gel filtration techniques. The enzyme had a molecular weight of 20.5 kDa on SDS-PAGE and was highly basic with a pI of 9.6. It was thermostable with a broad temperature optima for activity ranging from 37 to 70 degrees C and had an optimum pH of 5.2. Mercuric chloride and para-chloromercuric benzoate inhibited completely the enzyme while manganese chloride activated it. Antibodies raised against the purified beta-1,3-glucanase identified another protein with an apparent molecular weight of 30 kDa in western reactions. Significance of this enzyme in pearl millet-downy mildew host-pathogen interaction is discussed. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 06:28:06