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Titolo: Nondestructive, stereological estimation of plant root lengths, branchingpattern and diameter distribution
Autore: Wulfsohn, D; Nyengaard, JR; Gundersen, HJG; Cutler, AJ; Squires, TM;
 Indirizzi:
 Univ Saskatchewan, Dept Agr & Bioresource Engn, Saskatoon, SK S7N 5A9, Canada Univ Saskatchewan Saskatoon SK Canada S7N 5A9 skatoon, SK S7N 5A9, Canada Aarhus Univ, Stereol Res Lab, DK8000 Aarhus C, Denmark Aarhus Univ Aarhus Denmark C Stereol Res Lab, DK8000 Aarhus C, Denmark Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada Natl Res Council Canada Saskatoon SK Canada S7N 0W9 n, SK S7N 0W9, Canada
 Titolo Testata:
 PLANT AND SOIL
fascicolo: 12,
volume: 214,
anno: 1999,
pagine: 15  26
 SICI:
 0032079X(1999)214:12<15:NSEOPR>2.0.ZU;2V
 Fonte:
 ISI
 Lingua:
 ENG
 Soggetto:
 TOTAL VERTICAL PROJECTIONS; UNBIASED ESTIMATION; IMAGEANALYSIS; SCANNER; QUANTIFICATION; SECTIONS; CURVE;
 Keywords:
 Agropyron cristatum L .; growth curves; total vertical projections; unbiased stereology; variability;
 Tipo documento:
 Article
 Natura:
 Periodico
 Settore Disciplinare:
 Agriculture,Biology & Environmental Sciences
 Citazioni:
 33
 Recensione:
 Indirizzi per estratti:
 Indirizzo: Wulfsohn, D Univ Saskatchewan, Dept Agr & Bioresource Engn, 57 Campus Dr, Saskatoon, SK S7N 5A9, Canada Univ Saskatchewan 57 Campus Dr Saskatoon SK Canada S7N 5A9 ada



 Citazione:
 D. Wulfsohn et al., "Nondestructive, stereological estimation of plant root lengths, branchingpattern and diameter distribution", PLANT SOIL, 214(12), 1999, pp. 1526
Abstract
The total length of a linear structure contained in an unbounded, transparent reference space can be estimated from 'total vertical projections' obtained by rotating the linear structure about an arbitrary 'vertical' axis, and projecting the linear structure onto a plane parallel to the axis of rotation. The total number of intersections between cycloid arcs with their minor axis perpendicular to the axis of rotation and the projected linear structure then provides an unbiased estimator of the total length of the structure. In this study, a stereological procedure based on the method of 'total vertical projections' was used to nondestructively estimate total root length, number of branches, diameter distribution and mean root diameter of crested wheatgrass plants (Agropyron cristatum L.) growing in a transparentmedium. Root lengths, diameters, and number of branching points of variousorders were determined at 3day intervals over a 4week growing period. The length estimator was very robust and efficient with sampling coefficientsof error usually less than 5% for a total of 50150 grid intersection counts over two projection directions per plant. Biological coefficients of variance for total length were between 3070%, and were largely related to variation in the extent of branching.
ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 16:04:37