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Titolo:
Time-lapsed confocal microscopy reveals temporal and spatial expression ofthe lysine epsilon-aminotransferase gene in Streptomyces clavuligerus
Autore:
Han, L; Khetan, A; Hu, WS; Sherman, DH;
Indirizzi:
Univ Minnesota, Dept Microbiol, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 robiol, Minneapolis, MN 55455 USA Univ Minnesota, Biol Process Technol Inst, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 l Inst, Minneapolis, MN 55455 USA Univ Minnesota, Dept Mat Sci & Chem Engn, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 m Engn, Minneapolis, MN 55455 USA
Titolo Testata:
MOLECULAR MICROBIOLOGY
fascicolo: 5, volume: 34, anno: 1999,
pagine: 878 - 886
SICI:
0950-382X(199912)34:5<878:TCMRTA>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
GREEN FLUORESCENT PROTEIN; BETA-LACTAM BIOSYNTHESIS; BACILLUS-SUBTILIS; CEPHALOSPORIN BIOSYNTHESIS; DNA; LOCALIZATION; SYNTHETASE; MARKER; ENZYME; REPLICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Sherman, DH Univ Minnesota, Dept Microbiol, Box 196 UFHC,1450 Mayo Mem Bldg, Minneapolis, MN 55455 USA Univ Minnesota Box 196 UFHC,1450 Mayo Mem BldgMinneapolis MN USA 55455
Citazione:
L. Han et al., "Time-lapsed confocal microscopy reveals temporal and spatial expression ofthe lysine epsilon-aminotransferase gene in Streptomyces clavuligerus", MOL MICROB, 34(5), 1999, pp. 878-886

Abstract

To investigate the temporal and spatial expression patterns of the gene (lat) encoding lysine epsilon-aminotransferase (LAT) for cephamycin C biosynthesis, a mutant form of green fluorescent protein (mut1GFP) was integrated into the Streptomyces clavuligerus chromosome (strain LH369), resulting in a translational fusion with lat. LAT activity and fluorescence profiles of the recombinant protein paralleled the native LAT enzyme activity profile in wild-type S, clavuligerus, which peaked during exponential growth phase and decreased slowly towards stationary phase. These results indicate that the LAT-Mut1GFP fusion protein retains both LAT and GFP functionality in S, clavuligerus LH369. LH369 produced wild-type levels of cephamycin C in minimal medium culture conditions supplemented with lysine. Time-lapsed confocal microscopy of the S. clavuligerus LH369 strain revealed the temporal and spatial characteristics of laf gene expression and demonstrated that physiological development of S. clavuligerus colonies leading to cephamycin C biosynthesis is limited to the substrate mycelia.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 00:45:39