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Titolo:
Mitogenic effect of nerve growth factor (NGF) in LNCaP prostate adenocarcinoma cells: Role of the high- and low-affinity NGF receptors
Autore:
Sortino, MA; Condorelli, F; Vancheri, C; Chiarenza, A; Bernardini, R; Consoli, U; Canonico, PL;
Indirizzi:
Univ Catania, Sch Med, Inst Pharmacol, I-95125 Catania, Italy Univ Catania Catania Italy I-95125 nst Pharmacol, I-95125 Catania, Italy Univ Catania, Sch Med, Inst Resp Dis, I-95125 Catania, Italy Univ CataniaCatania Italy I-95125 Inst Resp Dis, I-95125 Catania, Italy Univ Catania, Sch Med, Inst Hematol, I-95125 Catania, Italy Univ Catania Catania Italy I-95125 Inst Hematol, I-95125 Catania, Italy Univ Pavia, Sch Med, Dept Internal Med, Pharmacol Sect, I-27100 Pavia, Italy Univ Pavia Pavia Italy I-27100 Med, Pharmacol Sect, I-27100 Pavia, Italy
Titolo Testata:
MOLECULAR ENDOCRINOLOGY
fascicolo: 1, volume: 14, anno: 2000,
pagine: 124 - 136
SICI:
0888-8809(200001)14:1<124:MEONGF>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
NORMAL RAT PROSTATE; FACTOR-LIKE PROTEIN; GENE-EXPRESSION; CELLULAR-LOCALIZATION; EPITHELIAL-CELLS; CANCER CELLS; FACTOR-I; LINE; TRK; INSULIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Sortino, MA Univ Catania, Sch Med, Inst Pharmacol, Viale Andrea Doria 6, I-95125 Catania, Italy Univ Catania Viale Andrea Doria 6 Catania Italy I-95125 Italy
Citazione:
M.A. Sortino et al., "Mitogenic effect of nerve growth factor (NGF) in LNCaP prostate adenocarcinoma cells: Role of the high- and low-affinity NGF receptors", MOL ENDOCR, 14(1), 2000, pp. 124-136

Abstract

We have investigated the effect of nerve growth factor (NGF) in the androgen-dependent, prostate adenocarcinoma LNCaP cell line. Exposure of LNCaP cells to NGF resulted in a significant increase of cell proliferation. The effect was concentration dependent and equally present in serum- or charcoal-stripped serum-supplemented and serum-deprived conditions. The mitogenic action of NGF was accompanied by an enhanced expression of prostate-specific antigen (PSA) and resulted additive to the proliferative effect of dihydrotestosterone. The proliferative effect of NGF appeared to be mediated by thehigh-affinity NGF receptor, p140(trka). Only p140(trka), but not the low-affinity NGF receptor, p75(LNGFR), was expressed in LNCaP cells; both the proliferative response and the phosphorylation of p140(trka) upon NGF treatment were prevented by the tyrosine kinase inhibitor K252a. LNCaP cells transiently transfected with the cDNA encoding for p75(LNGFR) appeared more sensitive to NGF, as demonstrated by the increased number of p75(LNGFR)-transfected LNCaP cells exposed for 72 h to NGF compared with wild LNCaP cultures. However, p75(LNGFR)-transfected LNCaP cells rapidly underwent apoptotic death when deprived of NGF. Our study demonstrates the physiological relevance of NGF in the regulation of prostate cell proliferation and the relative contribution of the high- end low-affinity NGF receptors in this control.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:31:11