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Titolo:
Isolation and characterization of AtMLH1, a MutL homologue from Arabidopsis thaliana
Autore:
Jean, M; Pelletier, J; Hilpert, M; Belzile, F; Kunze, R;
Indirizzi:
Univ Laval, Dept Phytol, Quebec City, PQ G1K 7P4, Canada Univ Laval Quebec City PQ Canada G1K 7P4 Quebec City, PQ G1K 7P4, Canada Univ Munich, Inst Genet & Mikrobiol, D-80638 Munich, Germany Univ Munich Munich Germany D-80638 & Mikrobiol, D-80638 Munich, Germany
Titolo Testata:
MOLECULAR AND GENERAL GENETICS
fascicolo: 4-5, volume: 262, anno: 1999,
pagine: 633 - 642
SICI:
0026-8925(199912)262:4-5<633:IACOAA>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA MISMATCH REPAIR; NONPOLYPOSIS COLON-CANCER; CROSSING-OVER; SACCHAROMYCES-CEREVISIAE; COLORECTAL-CANCER; MUTATIONS; GENE; MSH2; RECOMBINATION; MEIOSIS;
Keywords:
DNA mismatch repair; MutL homologue (MLH); T-DNA insertion; microsatellite; reverse genetics;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Kunze, R Univ Laval, Dept Phytol, Pavillon Charles Eugene Marchant, QuebecCity, PQG1K 7P4, Canada Univ Laval Pavillon Charles Eugene Marchant QuebecCity PQ Canada G1K 7P4
Citazione:
M. Jean et al., "Isolation and characterization of AtMLH1, a MutL homologue from Arabidopsis thaliana", MOL G GENET, 262(4-5), 1999, pp. 633-642

Abstract

DNA mismatch repair systems play an essential role in the maintenance of genetic information in living organisms and are also implicated in genetic recombination and genome stability. Using degenerate primers, we have clonedthe first plant homologue of the E. coli MutL gene, which we have called AtMLH1 for Arabidopsis thaliana MutL-homologue 1. AtMLH1 is present as a single-copy gene in the Arabidopsis genome and is located on the top arm of chromosome 4. Sequence analysis revealed that the product of this gene shows extensive sequence homology with other eukaryotic MLH1 proteins. As mlh1-deficient lines would be useful for studying the biological function of this gene, several populations that had been mutagenized using T-DNA and transposon insertions were screened to identify such mutants. One line that carries a T-DNA insertion in the promoter region of the AtMLH1 gene was isolated. Surprisingly, although the insertion occurred only approximate to 80 bp upstream of the putative transcription start site, Northern analyses revealedvery low but similar amounts of AtMLH1 transcript in both the wild type and the T-DNA insertion lines. RT-PCR analyses suggest, however, that transcription is initiated further upstream in the insertion line and that the T-DNA may supply this novel initiation site. Finally, no increase in microsatellite instability - a phenotype often associated with mutations in mismatchrepair genes - was observed in plants homozygous for this insertion.

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Documento generato il 29/11/20 alle ore 23:50:37