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Titolo:
DETECTION OF DNA POLYMORPHISMS IN THE GENUS KALANCHOE BY RAPD-PCR FINGERPRINT AND ITS RELATIONSHIPS TO INFRAGENERIC TAXONOMIC POSITION AND ECOPHYSIOLOGICAL PHOTOSYNTHETIC BEHAVIOR OF THE SPECIES
Autore:
GEHRIG HH; ROSICKE H; KLUGE M;
Indirizzi:
TECH UNIV DARMSTADT,INST BOT,SCHNITTSPAHNSTR 10 D-64287 DARMSTADT GERMANY
Titolo Testata:
PLANT SCIENCE
fascicolo: 1, volume: 125, anno: 1997,
pagine: 41 - 51
SICI:
0168-9452(1997)125:1<41:DODPIT>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
CRASSULACEAN ACID METABOLISM; ARBITRARY PRIMERS; GENETIC-VARIATION; JUNIPERUS; MARKERS; PLANT;
Keywords:
DELTA-C-13 VALUES; ECOPHYSIOLOGY; KALANCHOE; PHYLOGENY; RANDOMLY AMPLIFIED POLYMORPHIC DNA; TAXONOMY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
H.H. Gehrig et al., "DETECTION OF DNA POLYMORPHISMS IN THE GENUS KALANCHOE BY RAPD-PCR FINGERPRINT AND ITS RELATIONSHIPS TO INFRAGENERIC TAXONOMIC POSITION AND ECOPHYSIOLOGICAL PHOTOSYNTHETIC BEHAVIOR OF THE SPECIES", PLANT SCI, 125(1), 1997, pp. 41-51

Abstract

The genus Kalanchoe plays an important role in the investigation of biochemical, ecophysiological and evolutionary aspects of Crassulacean acid metabolism (CAM), an adaptationally relevant modification of the photosynthetic carbon assimilation pathway. In order to obtain more information to answer the question whether or not within the genus the modality of CAM performance is related to phylogenetic trends, we have applied RAPD-PCR analysis to compare 30 selected Kalanchoe species with ten 10-mer synthetic oligonucleotides having been used to amplify multiple PCR products with genomic DNA as template. The different plantswere scored for presence and absence of defined RAPD-fragments and grouped by distance analysis using a simple matching coefficient of similarity. Four primers which amplified between eight and 16 distinct fragments (52 informative sites building an average of 13 bands) were finally chosen for data analysis allowing us to generate dendrograms withthe 'neighbor joining' - and UPGMA-methods. Both methods resulted in nearly identical dendrograms showing that within the genus Kalanchoe the species form three main clusters which coincide well with the threeinfrageneric groups of species distinguishable by their mode of CAM performance and by classical taxonomic criteria. In contrast to the current critical discussion of the applicability of thee RRAPD-fingerprinting method for taxonomical studies our results suggest that RAPD technology is in fact a rapid and sensitive technique providing a useful tool for studying plant phylogenetic relationships at the infragenetic level. (C) 1997 Elsevier Science Ireland Ltd.

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Documento generato il 07/08/20 alle ore 12:19:25