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Titolo:
Relative abundances, isolation and structure of phlebotomine microsatellites
Autore:
Day, JC; Ready, PD;
Indirizzi:
Nat Hist Museum, Dept Entomol, Mol Systemat Lab, London SW7 5BD, England Nat Hist Museum London England SW7 5BD emat Lab, London SW7 5BD, England
Titolo Testata:
INSECT MOLECULAR BIOLOGY
fascicolo: 4, volume: 8, anno: 1999,
pagine: 575 - 580
SICI:
0962-1075(199911)8:4<575:RAIASO>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIPTERA; LOCI; WASP; DNA; POPULATIONS; PSYCHODIDAE; HYMENOPTERA; FREQUENCY;
Keywords:
microsatellites; AAT-class repeats; Lutzomyia whitmani; phlebotomine sandflies; website;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Ready, PD Nat Hist Museum, Dept Entomol, Mol Systemat Lab, Cromwell Rd, London SW7 5BD, England Nat Hist Museum Cromwell Rd London England SW7 5BD 5BD, England
Citazione:
J.C. Day e P.D. Ready, "Relative abundances, isolation and structure of phlebotomine microsatellites", INSEC MOL B, 8(4), 1999, pp. 575-580

Abstract

Popular classes of microsatellites are not always abundant in insects or easily isolated from them. Dot-blot hybridizations demonstrated much variation in the relative abundance of four repeal: classes in four phlebotomine sandfly species. Only AAT-class repeats were specifically isolated from a phagemid library of Lutzomyia whitmani, even though other microsatellites hadsimilar abundances. An enrichment step would have targeted classes but wasomitted because relatively long flanking sequences were sought. All fourteen sandfly loci had a non-coding structure, and a minority of dipteran AAT-class repeats found in DNA databases and the literature were from exons, Therefore, this class should often provide neutral alleles for population studies. Perfect, not imperfect, AAT-class repeats were polymorphic in wild L.whitmani.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/10/20 alle ore 14:46:24