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Titolo:
Platelets induce sinusoidal endothelial cell apoptosis upon reperfusion ofthe cold ischemic rat liver
Autore:
Sindram, D; Porte, RJ; Hoffman, MR; Bentley, RC; Clavien, PA;
Indirizzi:
Duke Univ, Med Ctr, Dept Surg, Hepatobiliary & Liver Transplant Lab, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 Liver Transplant Lab, Durham, NC 27710 USA Duke Univ, Med Ctr, Dept Pathol, Hepatobiliary & Liver Transplant Lab, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 Liver Transplant Lab, Durham, NC 27710 USA
Titolo Testata:
GASTROENTEROLOGY
fascicolo: 1, volume: 118, anno: 2000,
pagine: 183 - 191
SICI:
0016-5085(200001)118:1<183:PISECA>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
TOTAL HEPATIC ISCHEMIA; HUMAN BLOOD-PLATELETS; P-SELECTIN; PRESERVATION INJURY; ACTIVATED PLATELETS; MYOCARDIAL-ISCHEMIA; TRANSFORMING GROWTH-FACTOR-BETA-1; LYMPHOCYTE ADHERENCE; ADHESION MOLECULES; EURO-COLLINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
69
Recensione:
Indirizzi per estratti:
Indirizzo: Clavien, PA Duke Univ, Med Ctr, Dept Surg, Hepatobiliary & Liver Transplant Lab, POB 3247, Durham, NC 27710 USA Duke Univ POB 3247 Durham NC USA 27710 7, Durham, NC 27710 USA
Citazione:
D. Sindram et al., "Platelets induce sinusoidal endothelial cell apoptosis upon reperfusion ofthe cold ischemic rat liver", GASTROENTY, 118(1), 2000, pp. 183-191

Abstract

Background & Aims: Sinusoidal endothelial cell (SEC) apoptosis is a central feature of reperfusion injury in liver transplantation. Platelet sequestration occurs after transplantation with possible deleterious effects. We tested the hypothesis that platelets mediate SEC apoptosis. Methods: Livers were perfused after 24 hours of cold preservation in University of Wisconsinsolution in an isolated perfused rat liver model. The perfusate contained isolated syngeneic red blood cells and purified platelets. Effects of inhibiting platelet adhesion on SEC apoptosis was tested using sialyl Lewis-X oligosaccharide (sLe(x)), a natural ligand of selectin adhesion molecules. Reperfusion injury was assessed by established markers of injury. Apoptosis was determined by TUNEL and electron microscopy. Results: A third of the circulating platelets was rapidly sequestered in the liver after reperfusion. This was associated with increased graft injury. Single platelets were adherent to sinusoidal lining without morphological or dynamic evidence of impairment of microcirculation. TUNEL staining revealed a 6-fold increase in the number of apoptotic SECs at 1 hour of reperfusion. No hepatocyte death orevidence of necrosis was detected up to 3 hours of reperfusion. Addition of sLe(x) inhibited adhesion and significantly reduced SEC apoptosis. Conclusions: Platelets cause SEC apoptosis upon reperfusion of liver grafts, Prevention of adhesion is protective.

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Documento generato il 05/04/20 alle ore 13:40:19