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Titolo:
HCO3- secretion in the rat colonic crypt is closely linked to Cl- secretion
Autore:
Geibel, JP; Singh, S; Rajendran, VM; Binder, HJ;
Indirizzi:
Yale Univ, Sch Med, Dept Internal Med, Sect Digest Dis, New Haven, CT 06520 USA Yale Univ New Haven CT USA 06520 Sect Digest Dis, New Haven, CT 06520 USA Yale Univ, Dept Surg, New Haven, CT 06510 USA Yale Univ New Haven CT USA 06510 Univ, Dept Surg, New Haven, CT 06510 USA Yale Univ, Dept Cellular & Mol Physiol, New Haven, CT 06510 USA Yale UnivNew Haven CT USA 06510 r & Mol Physiol, New Haven, CT 06510 USA
Titolo Testata:
GASTROENTEROLOGY
fascicolo: 1, volume: 118, anno: 2000,
pagine: 101 - 107
SICI:
0016-5085(200001)118:1<101:HSITRC>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
BICARBONATE SECRETION; ION-TRANSPORT; DISTAL COLON; RABBIT ILEUM; ABSORPTION; EXCHANGE; INVITRO; MUCOSA; CELLS; FLUID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Binder, HJ Yale Univ, Sch Med, Dept Internal Med, Sect Digest Dis, 333 Cedar St,89 LMP, New Haven, CT 06520 USA Yale Univ 333 Cedar St,89 LMP New Haven CT USA 06520 06520 USA
Citazione:
J.P. Geibel et al., "HCO3- secretion in the rat colonic crypt is closely linked to Cl- secretion", GASTROENTY, 118(1), 2000, pp. 101-107

Abstract

Background & Aims: The mechanism of colonic HCO3- secretion has not been established largely because of a lack of experimental methods for its detailed study, The present studies were designed to establish whether the isolated, perfused crypt of the rat distal colon is an excellent model to study HCO3- movement and the mechanism of colonic HCO3- secretion, Methods: HCO3- secretion was determined in isolated, microperfused crypts by measuring [HCO3-] by microcalorimetry on nanoliter samples, Results: Net HCO3- absorption was observed during lumen and bath perfusion with an HCO,3--Ringer solution, Vasoactive intestinal polypeptide (60 nmol/L), acetylcholine (100 nmol/L), or dibutyryl adenosine 3',5'-cyclic monophosphate (DBcAMP, 0.5 mmol/L) induced active HCO3- secretion that required bath but not lumen HCO3-/CO2. DBcAMP-stimulated HCO3- secretion was not affected by acetazolamide, an inhibitor of carbonic anhydrase, Removal of lumen Cl- did not alter DBcAMP-stimulated HCO3- secretion but reduced fluid secretion. DBcAMP-stimulated HCO3- secretion was closely linked to active Cl- secretion because HCO3- secretion was substantially reduced by removal of bath Cl-, by addition of bath bumetanide, an inhibitor of Na-K-2Cl cotransport and Cl- secretion, and by addition of lumen NPPB, a Cl- channel inhibitor, Conclusions: These studies establish that colonic crypt HCO3- secretion (1) is not a result of an apical membrane Cl--HCO3- exchange, (2) is tightly associated with Cl- secretion, and (3) primarily occurs via an apical membrane Cl- channel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 09:27:59