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Titolo:
Identifying clinically important Gram-negative anaerobes from the oral cavity
Autore:
Haraldsson, G; Holbrook, WP;
Indirizzi:
Univ Iceland, Fac Odontol, IS-101 Reykjavik, Iceland Univ Iceland Reykjavik Iceland IS-101 Odontol, IS-101 Reykjavik, Iceland
Titolo Testata:
EUROPEAN JOURNAL OF ORAL SCIENCES
fascicolo: 6, volume: 107, anno: 1999,
pagine: 429 - 436
SICI:
0909-8836(199912)107:6<429:ICIGAF>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
PREVOTELLA-INTERMEDIA; COMB-NOV; BACTEROIDES-ASACCHAROLYTICUS; GENUS PORPHYROMONAS; NIGRESCENS; RECLASSIFICATION; PROPOSAL; MOORE; MELANINOGENICUS; EMENDATION;
Keywords:
bacterial identification; polymerase chain reaction; periodontal pathogens; Prevotella sp; Porphyromonas gingivalis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Haraldsson, G Univ Iceland, Fac Odontol, Vatnsmyrarvegur 16, IS-101 Reykjavik, Iceland Univ Iceland Vatnsmyrarvegur 16 Reykjavik Iceland IS-101 nd
Citazione:
G. Haraldsson e W.P. Holbrook, "Identifying clinically important Gram-negative anaerobes from the oral cavity", EUR J OR SC, 107(6), 1999, pp. 429-436

Abstract

Gram-negative oral anaerobes have frequently been associated with periodontal disease, some species more frequently than others. The confusing classification of these organisms has often obscured the association with diseaseof particular species within this group of organisms. This investigation aimed to compare different identification methods that could be applied in clinical research. Clinical isolates were collected and identified by three different methods: screening with phenotypic tests, commercial identification kits, and a 16S rRNA-based polymerase chain reaction (PCR) method. Forty-three reference strains of 19 Prevotella and Porphyromonas species were also included in the investigation. The phenotypic screen easily differentiated Porph. gingivalis from the other pigmented species. The screen also gavea good indication of separation of the lactose-fermenting species from thelactose non-fermenting species, although diversity can be seen in beta-galactosidase activity. Commercial identification kits did not add much to identification achieved with the phenotypic screen, only 20% of Porph. gingivalis isolates could be identified to species level with the kits. Neither the kits nor the phenotypic screen could differentiate Pr. intermedia and Pr.nigrescens. With the PCR method, Pr. intermedia and Pr. nigrescens were easily separated, and Porph. gingivalis was readily identified. Because of 16S rRNA gene sequence similarity, Pr. melaninogenica and Pr. veroralis couldnot be separated by the PCR method.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 15:32:24