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Titolo:
Role of wound healing myofibroblasts on re-epithelialization of human skin
Autore:
Moulin, V; Auger, FA; Garrel, D; Germain, L;
Indirizzi:
Hop St Sacrement, Lab Grands Brules, LOEX, Quebec City, PQ G1S 4L8, CanadaHop St Sacrement Quebec City PQ Canada G1S 4L8 c City, PQ G1S 4L8, Canada
Titolo Testata:
BURNS
fascicolo: 1, volume: 26, anno: 2000,
pagine: 3 - 12
SICI:
0305-4179(200002)26:1<3:ROWHMO>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
BASEMENT-MEMBRANE FORMATION; NORMAL HUMAN KERATINOCYTES; GROWTH-FACTOR-BETA; DERMAL FIBROBLASTS; IN-VITRO; GRANULATION-TISSUE; DIFFERENTIATION; EXPRESSION;
Keywords:
wound healing; myofibroblast; keratinocyte; basement membrane; skin equivalent;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Moulin, V LOEX, 1050 Chemin St Foy, Quebec City, PQ G1S 4L8, Canada LOEX 1050 Chemin St Foy Quebec City PQ Canada G1S 4L8 L8, Canada
Citazione:
V. Moulin et al., "Role of wound healing myofibroblasts on re-epithelialization of human skin", BURNS, 26(1), 2000, pp. 3-12

Abstract

In human skin, large burned surfaces heal using two concomitant phenomena:re-epithelialization and dermal neoformation. Numerous studies report the role of interactions between keratinocytes and fibroblasts, but the relationship between wound healing myofibroblasts and keratinocytes is not clear, even though these two cell types coexist during healing. We investigated the influence of myofibroblasts on keratinocyte growth and differentiation using an in vitro skin model. A histological study was performed to determinethe speed and quality of epithelialization. When the dermis was populated with fibroblasts, a continuous epidermis was formed in 7-10 days. In contrast, with wound healing myofibroblasts or without cell in dermis, the complete re epithelialization never occurred over the 10-day period studied. After 7 further days of epidermal differentiation, histology showed an epidermis more disorganized and expression of basement membrane constituents was reduced when wound healing myofibroblasts or no cells were added in the dermis instead of fibroblasts. These results suggest that wound healing myofibroblasts are not efficient to stimulate keratinocyte growth and differentiation. Treatment of fibroblasts with TGF beta 1 induced an increase of epidermal cell differentiation as seen when myofibroblasts were present. However, this cytokine did not change re-epithelialization rate and induced an increase of basement membrane matrix deposition in opposition to myofibroblasts. Thus, TGF beta 1 action is not sufficient to explain all the different keratinocyte reactions towards fibroblasts and wound healing myofibroblasts. Our conclusion is that myofibroblasts seem to have a limited role in the re-epithelialization process and might be more associated with the increased extracellular matrix secretion. (C) 2000 Elsevier Science Ltd and ISBI. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 15:27:31