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Titolo:
Effects of progesterone receptor blockers on human granulosa-luteal cell culture secretion of progesterone, estradiol, and relaxin
Autore:
VandeVoort, CA; Overstreet, JW; Lasley, BL; Stewart, DR;
Indirizzi:
Univ Calif Davis, Calif Reg Primate Res Ctr, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 Primate Res Ctr, Davis, CA 95616 USA Univ Calif Davis, Div Reprod Biol, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 Div Reprod Biol, Davis, CA 95616 USA Univ Calif Davis, Dept Ob Gyn, Davis, CA 95616 USA Univ Calif Davis DavisCA USA 95616 vis, Dept Ob Gyn, Davis, CA 95616 USA Univ Calif Davis, Inst Toxicol & Environm Hlth, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 & Environm Hlth, Davis, CA 95616 USA
Titolo Testata:
BIOLOGY OF REPRODUCTION
fascicolo: 1, volume: 62, anno: 2000,
pagine: 200 - 205
SICI:
0006-3363(200001)62:1<200:EOPRBO>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRIMATE CORPUS-LUTEUM; MENSTRUAL-CYCLE; ANTIPROGESTERONE RU486; FOLLICULAR PHASE; NORMAL WOMEN; RU-486; DEHYDROGENASE; INHIBITION; LOCALIZATION; MECHANISM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: VandeVoort, CA Univ Calif Davis, Calif Reg Primate Res Ctr, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 r, Davis, CA 95616 USA
Citazione:
C.A. VandeVoort et al., "Effects of progesterone receptor blockers on human granulosa-luteal cell culture secretion of progesterone, estradiol, and relaxin", BIOL REPROD, 62(1), 2000, pp. 200-205

Abstract

We have developed culture methods for human luteinizing granulosa cells (GLC) that support the timely and dynamic secretion of estrogen (estradiol-17beta; E-2), progesterone (P-4), and relaxin (Rlx) in patterns that mimic serum hormone concentrations during the luteal phase of the menstrual cycle. Additional hCG, to simulate rescue of the corpus luteum, prevented the normal decline in GLC hormone production. To test the importance of the P-4 receptor in P-4 production, GLC were treated in vitro with two P-4 receptor antagonists. Human GLC received one of two hCG support protocols: a Baselinegroup simulating the normal luteal phase or a Rescue group simulating early pregnancy. Baseline and Rescue groups were treated with either RU486 or HRP2000 either early or late in the cell culture period. The effects of treatments or control on ovarian steroid and peptide hormone production were determined (significant difference was P < 0.05). In the Rescue group, late treatment resulted in an immediate and dramatic decline in E-2, P-4, and Rlxsecretion to nearly nondetectable revers within 1 day after treatment, andhormones remained depressed for the remaining 10 days of culture. In contrast, early treatment resulted in a decline in steroid hormone secretion that returned to control levels within 5 days of cessation of treatment, and Rlx secretion was delayed for approximately 5 days more than in controls. The data support the hypothesis that P-4 may be a required autocrine factor, not only for its own production but also for the maintenance of full endocrine function of the corpus luteum.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 18:52:06