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Titolo:
Molecular characterization of a mutable pigmentation phenotype and isolation of the first active transposable element from Sorghum bicolor
Autore:
Chopra, S; Brendel, V; Zhang, JB; Axtell, JD; Peterson, T;
Indirizzi:
Iowa State Univ, Dept Zool & Genet, Ames, IA 50011 USA Iowa State Univ Ames IA USA 50011 , Dept Zool & Genet, Ames, IA 50011 USA Iowa State Univ, Dept Agron, Ames, IA 50011 USA Iowa State Univ Ames IA USA 50011 te Univ, Dept Agron, Ames, IA 50011 USA Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA Purdue Univ W Lafayette IN USA 47907 ept Agron, W Lafayette, IN 47907 USA
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 26, volume: 96, anno: 1999,
pagine: 15330 - 15335
SICI:
0027-8424(199912)96:26<15330:MCOAMP>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAIZE-P-GENE; DNA-BINDING; ZEA-MAYS; ANTIRRHINUM-MAJUS; PLANT GENOMES; SEQUENCE; EN/SPM; PREDICTION; INSERTION; PROTEINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Peterson, T Iowa State Univ, Dept Zool & Genet, 2206 Mol Biol Bldg, Ames, IA 50011 USA Iowa State Univ 2206 Mol Biol Bldg Ames IA USA 50011 50011 USA
Citazione:
S. Chopra et al., "Molecular characterization of a mutable pigmentation phenotype and isolation of the first active transposable element from Sorghum bicolor", P NAS US, 96(26), 1999, pp. 15330-15335

Abstract

Accumulation of red phlobaphene pigments in sorghum grain pericarp is under the control of the Y gene. A mutable allele of Y, designated as y-cs (y-candystripe), produces a variegated pericarp phenotype. Using probes from the maize p1 gene that cross-hybridize with the sorghum Y gene, we isolated the y-cs allele containing a large insertion element. Our results show that the Y gene is a member of the MYB-transcription factor family. The insertion element, named Candystripe1 (Cs1), is present in the second intron of theY gene and shares features of the CACTA superfamily of transposons. Cs1 is23,018 bp in size and is bordered by 20-bp terminal inverted repeat sequences. It generated a 3-bp target site duplication upon insertion within the Y gene and excised from y-cs, leaving a 2-bp footprint in two cases analyzed. Reinsertion of the excised copy of Cs1 was identified by Southern hybridization in the genome of each of seven red pericarp revertant lines tested. Cs1 is the first active transposable element isolated from sorghum. Our analysis suggests that Cs1-homologous sequences are present in low copy number in sorghum and other grasses, including sudangrass, maize, rice, teosinte, and sugarcane. The low copy number and high transposition frequency of Cs1 imply that this transposon could prove to be an efficient gene isolation tool in sorghum.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 01:48:24