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Titolo:
A serotonin transporter gene intron 2 polymorphic region, correlated with affective disorders, has allele-dependent differential enhancer-like properties in the mouse embryo
Autore:
MacKenzie, A; Quinn, J;
Indirizzi:
Univ Edinburgh, Royal Dick Sch Vet Studies, Dept Vet Pathol, Edinburgh EH91QH, Midlothian, Scotland Univ Edinburgh Edinburgh Midlothian Scotland EH91QH Midlothian, Scotland
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 26, volume: 96, anno: 1999,
pagine: 15251 - 15255
SICI:
0027-8424(199912)96:26<15251:ASTGI2>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEVELOPING RAT-BRAIN; FUNCTIONAL POLYMORPHISM; TRANSGENIC MICE; NERVOUS-SYSTEM; MESSENGER-RNA; ASSOCIATION; EXPRESSION; NEURONS; SUSCEPTIBILITY; GROWTH;
Keywords:
transcriptional regulation; promoter; transgenic mice; LacZ gene; rostral hindbrain;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Quinn, J Univ Edinburgh, Royal Dick Sch Vet Studies, Dept Vet Pathol, Edinburgh EH91QH, Midlothian, Scotland Univ Edinburgh Edinburgh Midlothian Scotland EH9 1QH n, Scotland
Citazione:
A. MacKenzie e J. Quinn, "A serotonin transporter gene intron 2 polymorphic region, correlated with affective disorders, has allele-dependent differential enhancer-like properties in the mouse embryo", P NAS US, 96(26), 1999, pp. 15251-15255

Abstract

Polymorphic regions consisting of a variable number of tandem repeats within intron 2 of the gene coding for the serotonin transporter protein 5-HTT have been associated with susceptibility to affective disorders. We have cloned two of these intronic polymorphisms. Stin2.10 and Stin2.12, into an expression vector containing a heterologous minimal promoter and the bacterial LacZ reporter gene. These constructs were then used to produce transgenicmice. In embryonic day 10.5 embryos, both Stin2.10 and Stin2.12 produced consistent beta-galactosidase expression in the embryonic midbrain, hindbrain, and spinal cord floor plate. However, we observed that the levels of beta-galactosidase expression produced by both the Stin2.10 and Stin2.12 within the rostral hindbrain differed significantly at embryonic day 10.5. Our data suggest that these polymorphic variable number of tandem repeats regions act as transcriptional regulators and have allele-dependent differential enhancer-like properties within an area of the hindbrain where the 5-HTT gene is known to be transcribed at this stage of development.

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Documento generato il 05/04/20 alle ore 02:39:49