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Titolo:
Magnocellular neurons of the rat supraoptic nucleus are endowed with functional nicotinic acetylcholine receptors
Autore:
Zaninetti, M; Blanchet, C; Tribollet, E; Bertrand, D; Raggenbass, M;
Indirizzi:
Univ Geneva, Ctr Med, Dept Physiol, CH-1211 Geneva 4, Switzerland Univ Geneva Geneva Switzerland 4 Physiol, CH-1211 Geneva 4, Switzerland
Titolo Testata:
NEUROSCIENCE
fascicolo: 2, volume: 95, anno: 2000,
pagine: 319 - 323
SICI:
0306-4522(2000)95:2<319:MNOTRS>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
BINDING-SITES; ALPHA-7 RECEPTOR; XENOPUS OOCYTES; CELLS; BUNGAROTOXIN; HYPOTHALAMUS; RECTIFICATION; VASOPRESSIN; CALCIUM; RELEASE;
Keywords:
autoradiography; brain slices; hypothalamus; vasopressin; dihydro-beta-erythroidine; whole-cell recordings;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Raggenbass, M Univ Geneva, Ctr Med, Dept Physiol, CH-1211 Geneva 4, Switzerland Univ Geneva Geneva Switzerland 4 211 Geneva 4, Switzerland
Citazione:
M. Zaninetti et al., "Magnocellular neurons of the rat supraoptic nucleus are endowed with functional nicotinic acetylcholine receptors", NEUROSCIENC, 95(2), 2000, pp. 319-323

Abstract

Acetylcholine can stimulate the release of vasopressin.(25) In organ-cultured hypothalamo-neurohypophyseal systems, acetylcholine enhanced vasopressin release by acting in or near the supraoptic nucleus.(28) Extracellular recordings(3,9,13) suggested that acetylcholine can increase supraoptic neuron excitability, These effects could be mimicked, in part, by nicotine or blocked by nicotinic antagonists, suggesting that they might be mediated by nicotinic acetylcholine receptors, Autoradiography indicated that alpha-bungarotoxin binding sites are present in the supraoptic nucleus;(8,14,19,27) however, neither acetylcholine nor nicotine binding sites could be detected.(8,14,27) Thus, the existence, let alone the nature, of nicotinic receptorsin the supraoptic nucleus has so far remained elusive. The present work attempts to determine: (i) whether functional nicotinic receptors are presentin this nucleus; (ii) whether they are located on neurosecretory magnocellular cells or at presynaptic sites; (iii) what their pharmacological and biophysical properties are; (iv) whether they influence the activity of all or only part of supraoptic neurons. Whole-cell recordings were performed in hypothalamic slices or in acutely dissociated supraoptic neurons and the effect of nicotinic agonists was tested under voltage-clamp conditions. Autoradiography was done in coronal hypothalamic sections, using [H-3]epibatidine and [I-125]alpha-bungarotoxin as ligands, Our results indicate that supraoptic neurons possess functional nicotinic receptors containing the alpha 7subunit, (C) 1999 IBRO, Published by Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 21:38:46