Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Family 19 chitinases of Streptomyces species: characterization and distribution
Autore:
Watanabe, T; Kanai, R; Kawase, T; Tanabe, T; Mitsutomi, M; Sakuda, S; Miyashita, K;
Indirizzi:
Niigata Univ, Fac Agr, Dept Appl Biol Chem, Niigata 9502181, Japan NiigataUniv Niigata Japan 9502181 ppl Biol Chem, Niigata 9502181, Japan Saga Univ, Fac Agr, Dept Appl Biol Sci, Saga 8408502, Japan Saga Univ Saga Japan 8408502 gr, Dept Appl Biol Sci, Saga 8408502, Japan Univ Tokyo, Dept Appl Biol Chem, Bunkyo Ku, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 iol Chem, Bunkyo Ku, Tokyo 1138657, Japan Natl Inst Agroenvironm Sci, Tsukuba, Ibaraki 3058604, Japan Natl Inst Agroenvironm Sci Tsukuba Ibaraki Japan 3058604 i 3058604, Japan
Titolo Testata:
MICROBIOLOGY-UK
, volume: 145, anno: 1999,
parte:, 12
pagine: 3353 - 3363
SICI:
1350-0872(199912)145:<3353:F1COSS>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACID-SEQUENCE SIMILARITIES; BACILLUS-CIRCULANS WL-12; CYSTEINE-RICH DOMAIN; GLYCOSYL HYDROLASES; PLANT CHITINASES; ANTIFUNGAL PROPERTIES; CRYSTAL-STRUCTURE; GRISEUS HUT-6037; PROTEINS; ENDOCHITINASE;
Keywords:
chitinase; Streptomyces; allosamidin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Watanabe, T Niigata Univ, Fac Agr, Dept Appl Biol Chem, 8050 Ikarashi-2, Niigata 9502181, Japan Niigata Univ 8050 Ikarashi-2 Niigata Japan 9502181 181, Japan
Citazione:
T. Watanabe et al., "Family 19 chitinases of Streptomyces species: characterization and distribution", MICROBIO-UK, 145, 1999, pp. 3353-3363

Abstract

Chitinase C from Streptomyces griseus HUT6037, described in 1997, is the first family 19 chitinase found in an organism other than higher plants, In this study, some properties of chitinase C were compared with those of family 18 bacterial chitinases, and the distribution of family 19 chitinases inStreptomyces species was investigated, The specific hydrolysing activity of chitinase C against soluble and insoluble chitinous substrates was markedly higher than those of bacterial family 18 chitinases. Chitinase C exhibited marked antifungal activity, whereas the other bacterial chitinases examined had no antifungal activity, Chitinase C was insensitive to allosamidin,whereas the family 18 bacterial chitinases were sensitive. Taking advantage of this insensitivity to allosamidin, a search was made for family 19 chitinases in University of Tokyo, various Streptomyces species, Chitinases insensitive to allosamidin were detected in the culture supernatants of all tested Streptomyces species. Southern hybridization analysis using a labelled DNA fragment corresponding to the catalytic domain of chitinase C strongly suggested that these species have genes similar to the chiC gene of S. griseus HUT6037, DNA fragments corresponding to the major part of the catalytic domains were amplified by PCR. The amplified fragments encoded amino acid sequences very similar to that of the corresponding region of chitinase C, Therefore, it was concluded that Streptomyces species generally possess family 19 chitinases which are very similar to chitinase C, Comparison of their amino acid sequences with those of plant family 19 chitinases revealed that Streptomyces family 19 chitinases are class IV type in terms of the presence and positions of deletions of amino acid sequences which are characteristic of plant class IV chitinases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 01:29:26